Novel human embryo kidney 293 cell HEK293ar of mammal engineering cell subset

Abstract

The invention provides a novel human embryo kidney 293 cell HEK293ar of a mammal engineering cell subset and application thereof. The novel human embryo kidney 293 cell HEK293ar of the mammal engineering cell subset is preserved by the China Center for Type Culture Collection (CCTCC) and the preservation number is CCTCC NO: C200927. The novel human embryo kidney 293 cell HEK293ar of the mammal engineering cell subset can be used for high-efficiency transient transfection exogenous genes and can grow in the form of a cell mass. A cell subset provided by the invention has the characteristics of suspension adaptable growth and adherent growth at the same time. The novel human embryo kidney 293 cell HEK293ar of the mammal engineering cell subset can overcome the defects that a gene expression product has short period and low yield when mammal engineering adherent cells are used for culturing and producing medicaments at present, and the efficiency for a suspension cell to perform transient transfection of exogenous genes is low. The novel human embryo kidney 293 cell HEK293ar of the mammal engineering cell subset can be used for establishing a novel scale transient high-efficiency transfection system for producing pharmaceutical proteins in an adherent transfection-suspension culture mode, and establishing an economic vector-free immobilized culture production mode by using the cell mass as a growth pattern.

Description

technical field [0001] The invention relates to a mammalian engineering cell suspension adaptation-adhesive cell subgroup; in particular, a novel mammalian engineering cell subgroup human embryonic kidney 293 cell HEK293ar and its application, which can be applied to large-scale transient transfection and non-toxic Vectorized cell mass culture to produce recombinant adenovirus vector and recombinant protein. Background technique [0002] Currently commonly used engineered cells, such as HEK293, CHO, BHK, etc., generally undergo apoptosis or cycle arrest in suspension culture. In engineering, the suspension adaptation method is often used to adapt the adherent cells to obtain adaptive cells suitable for large-scale suspension culture, because only the suspension culture method is more conducive to industrial scale cultivation. The suspension adaptation process of animal engineered cells generally goes through several months or even adult repeated cycles of adherence and susp...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a human embryonic kidney 293 cell subpopulation with the characteristics of suspension adaptation culture and adherent growth characteristics in order to overcome the specific problems faced in the production of protein drugs by mammalian engineering cells at present

The acquisition and use of this subpopulation helps to establish a novel animal engineering cell transient transfection process, which solves the problem of low transfection and protein production efficiency in the process of large-scale transient transfection of suspension-adapted cells to produce pharmaceutical proteins

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