Use of baicalin in preparation of targeted organ protection medicaments
A technology of baicalin and application, applied in the new application field of baicalin in the preparation of medicaments for preventing and treating allergic rhinitis
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Embodiment 1
[0026] Example 1 Baicalin antagonizes the increase in permeability and exudation of the nasal mucosa of sensitized rats in vivo
[0027] Experimental method: 250g male Brown Norway (BN) rats were divided into normal control group, model control group, baicalin group, and total glucosides of paeony. Normal group, model group, and baicalin group n=7; total glucosides of paeony group n=5.
[0028] BN rats in each group were sensitized by intraperitoneal injection of 1 mg ovalbumin (OVA) 1 mg, 0.5 ml of aluminum hydroxide gel and 1 ml of normal saline suspension. Seven days later, the same method was used to strengthen the sensitization once. The rats in the normal control group were intraperitoneally injected with saline. On the 8th day after the animals were sensitized, the rats in the medication group were given the drug suspension by gavage (40 mg / kg of baicalin and 70 mg / kg of total glucosides of paeony) once a day, 2 ml each time, for 12 days. The rats in the normal control gro...
Embodiment 2
[0030] Example 2 Baicalin inhibits the proliferation of splenic lymphocytes in vivo sensitized mice
[0031] Experimental method: BALB / C male and 6-week-old mice were randomly divided into 3 groups: normal group, model group and baicalin group. Normal group and baicalin group n=17; model group n=15.
[0032] Mice were sensitized by intraperitoneal injection of 100μg of OVA and 0.3ml of aluminum hydroxide gel mixture on Day0. The same method was used to enhance sensitization twice on Day7 and Day14. From Day 21 to Day 27, 20 μl of 2.5% OVA physiological saline solution was given daily for nasal challenge. The normal group was given intraperitoneal injection of saline and nose drops in the same way as the model group. The mice in the baicalin group were administered intragastrically from Day0, the dosage was 40mg / Kg, and ended on Day27. Each mouse was given 0.5ml gavage daily, and 0.5ml drinking water was given to mice in the normal group and model group.
[0033] The mice were ane...
Embodiment 3
[0037] Example 3 The dose-effect relationship of baicalin inhibiting the proliferation of splenic lymphocytes in vivo sensitized rats
[0038] Experimental method: dissolve baicalin powder in sterilized water and dissolve baicalin powder in a small amount of DMSO, adjust the pH to 7.0, and then use RPMI-1640 to make the volume constant, the concentration is 10mg / ml, and the DMSO content is less than 1‰. After filtering and sterilizing the above baicalin solution through a 0.2um microfilter, it is diluted to 0.0625, 0.125, 0.25, 0.5, 1.0g / L with RPMI-1640 times ratio.
[0039] 6 male SD rats of 250g were sensitized by intraperitoneal injection of 1ml of ovalbumin (OVA) 1mg, 0.5ml of aluminum hydroxide gel and 1ml of normal saline suspension. Seven days later, the same method was used to strengthen the sensitization once to 28 Animals were sacrificed under anesthesia for the first day, the spleen was taken, cut into pieces, and the spleen lymphocytes were separated with Ficoll Lympho...
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