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Xylaria gracillima strain, liquid fermentation and culture method thereof and application

A kind of carbon horn bacteria, small technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of not finding tiny carbon horn bacteria, achieve good economic and social benefits, simple raw materials, and high yield high rate effect

Inactive Publication Date: 2011-07-06
北京昊瑞康源生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the artificial fermentation and cultivation of C. pumilus to utilize the product

Method used

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  • Xylaria gracillima strain, liquid fermentation and culture method thereof and application
  • Xylaria gracillima strain, liquid fermentation and culture method thereof and application
  • Xylaria gracillima strain, liquid fermentation and culture method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the production of Xylocarpin microcystis mycelium

[0043] Under the culture condition that is most suitable for the growth of Pyrocystis microbes, the present invention obtains the medium formula (hereinafter referred to as fine charcoal) that is suitable for the growth of Pyremycetes microbes through the screening and comparison of the substratum formula and that the mycelium yield is higher. horn fungus preferred medium).

[0044] Optimal medium for C. parvicularis 1: 20.0 grams of soybean powder (commercially available), 50.0 grams of maltodextrin (Shandong Baolingbao Biotechnology Co., Ltd.), 5.0 grams of yeast extract (Shanghai Ridgelight Yeast Products Co., Ltd., catalog number : 000112), 3.0 grams of magnesium sulfate (Xilong Chemical Factory, Shantou, Guangdong), and dilute to 1000mL with water.

[0045] Optimal medium for C. microbes 2: 10.0 grams of soybean powder, 50.0 grams of glucose (Beijing Modern Oriental Fine Chemicals Co., Ltd.), 5.0 gr...

Embodiment 2

[0056] Embodiment 2, the processing of fermentation product and mycelia extraction

[0057] Fermentation broth treatment: Fermentation broth contains a large number of metabolites including exopolysaccharides, vitamins, amino acids, etc., which have a special aroma. Concentrate the fermented liquid in vacuum at a low temperature of 40-50°C to keep the activity of the metabolites, freeze-dry it into powder, and keep it for later use. Store away from light, dry and at room temperature.

[0058] Mycelium treatment: filter with gauze to obtain mycelium and fermentation liquid, wash mycelium with distilled water 2-3 times, freeze and vacuum dry, grind into powder, and set aside. Store away from light, dry and at room temperature.

Embodiment 3

[0059] Embodiment 3, the antioxidant activity detection of mycelia extract

[0060] Oxygen free radicals and their derivatives H 2 o 2 , lipid peroxide (LOOH) and singlet oxygen are collectively referred to as reactive oxygen species (Reactive Oxygen Species, ROS) (Mathew & Abraham, 2006). It refers to certain products of oxygen and oxygen-containing products of some reactions. It is characterized by containing oxygen, and its chemical properties are more active than oxygen. ROS can produce a series of harmful effects on biological organisms (Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Crit Rev FoodSci., 2004, 44: 275-295). Oxidative damage is closely related to the occurrence of many diseases (Jacob, R.S., & Burri, B.J. Oxidative damage and defense. American Journal of Clinical Nutrition, 1996, 63: 985S-990S), due to the damage of free radicals, that is, reactive oxygen species, neuropathy can occur (Floyd, R.A. Neuroinammatory process...

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Abstract

The invention relates to an xylaria gracillima strain CGMCC NO. 2449 separated from a termite abandoned nest, a liquid fermentation and culture method thereof and application of a mycelium extract.

Description

technical field [0001] The invention relates to a strain CGMCC No.2449 of a rare medicinal fungus Xylaria gracillima (Fr.) Fr.] and a liquid fermentation culture method thereof. The strains were submerged in submerged fermentation with appropriate medium, and the antioxidant activity of the fermentation products was identified. Background technique [0002] The strain of Xylaria gracillima was isolated and identified in termite waste nest by the Institute of Microbiology, Chinese Academy of Sciences on June 2, 2005, and the Chinese name of the strain was named by the Institute of Microbiology, Chinese Academy of Sciences. It was deposited on April 14, 2008 in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, China, 100101), and the deposit registration number is: CGMCC No.2449. Xylaria microbes belong to the subphylum Ascomycotin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02C12R1/645
Inventor 文华安李赛飞
Owner 北京昊瑞康源生物技术发展有限公司
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