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Synthetic medium of broad-leave Sparassis crispa

A technology for synthesizing culture media and hydrangea bacteria, applied in the direction of fungi, etc., can solve the problems of difficult downstream processing and complex media, and achieve the effect of no heavy metal pollution, simple process and easy acquisition

Active Publication Date: 2014-03-12
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent application number: 201110220578.4), but the culture medium is relatively complex, requiring the use of pine needles, composite carbon sources, etc., the raw materials will be subject to certain restrictions, and the complex components will bring difficulties to downstream processing

Method used

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  • Synthetic medium of broad-leave Sparassis crispa

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, utilize synthetic culture medium fermentation to produce hydrangea mycelium

[0037] Hydrangea synthetic medium formula is: 30.0 grams of soluble starch (converted to 12 grams of carbon), 3.18 grams of ammonium citrate (converted to 0.54 grams of nitrogen), 2.0 grams of potassium dihydrogen phosphate, 1.0 grams of magnesium sulfate, vitamin B 1 0.1 g, dilute to 1000 ml with water, adjust the pH value to 4.0, subpackage and sterilize the medium.

[0038] Control medium 1: 200 grams of potatoes, 20.0 grams of glucose, natural pH, and dilute to 1000 ml with water.

[0039] Control medium 2: 200 grams of potatoes, 40.0 grams of brown sugar, 3.0 grams of peptone, 3.0 grams of soybean oil, KH 2 PO 4 3.0g, MgSO 4 1.5g, 100ml of pine needle juice, dilute to 1000mL with water, adjust pH to 4.0. Potato processing: Peel the potatoes, cut into small pieces and boil for half an hour, then filter through gauze to get potato juice. Preparation of pine needle juice:...

Embodiment 2

[0048] Embodiment 2, utilize synthetic culture medium fermentation to produce hydrangea mycelium

[0049] Hydrangea synthetic medium formula is: 20.0 grams of glucose (converted to 8 grams of carbon), 2.12 grams of ammonium citrate (converted to 0.36 grams of nitrogen), 0.5 grams of potassium dihydrogen phosphate, 1.0 grams of magnesium sulfate, vitamin B 1 0.05 g, dilute to 1000 ml with water, adjust the pH value to 3.0, and sterilize the culture medium. Control medium: 200 grams of potatoes, 20.0 grams of glucose, natural pH, dilute to 1000 ml with water.

[0050] Hydrangea strains (original number: Hydrangea No. 18; purchased from Mianyang Edible Fungi Research Institute, Sichuan Province) were inoculated on potato solid medium (PDA) for activation. The culture temperature was 20-28°C and the culture time was 14 days. Under the initial pH value of 6.0, the hydrangea strains were inoculated in the above-mentioned hydrangea synthetic medium according to the inoculum amount o...

Embodiment 3

[0054] Embodiment 3, utilize synthetic culture medium fermentation to produce hydrangea mycelium

[0055] Hydrangea synthetic medium formula is: 62.5 grams of maltose (converted to 25 grams of carbon), 9.4 grams of diammonium hydrogen phosphate (converted to 1.99 grams of nitrogen), 1.0 grams of potassium dihydrogen phosphate, vitamin B 1 0.05 g, dilute to 1000 ml with water, adjust the pH value to 6.0, and sterilize the culture medium. Control medium: 200 grams of potatoes, 20.0 grams of glucose, natural pH, dilute to 1000 ml with water.

[0056] Hydrangea strains (original number: Hydrangea No. 18; purchased from Mianyang Edible Fungi Research Institute, Sichuan Province) were inoculated on potato solid medium (PDA) for activation. The culture temperature was 20-28°C and the culture time was 14 days. Under the initial pH value of 6.0, the hydrangea strains were inoculated in the above-mentioned hydrangea synthetic medium according to the inoculum amount of 10%, and then sha...

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Abstract

The invention discloses a synthetic medium of broad-leave Sparassis crispa. The synthetic medium comprises carbon, nitrogen, potassium phosphate monobasic, other component and water. The other component is at least one of magnesium sulfate and vitamin B1. The final concentration of the carbon, the nitrogen, the potassium phosphate monobasic, the magnesium and the vitamin B1 in the synthetic medium is sequentially 8.0-25.0g / L, 0.15-4.5g / L, 0.5-2.0g / L, 0-1.0g / L and 0-0.1g / L. The carbon comes from sucrose or glucose or maltose or lactose or soluble starch. The nitrogen comes from ammonium citrate or ammonium nitrate or ammonium phosphate or potassium nitrate or ammonium sulfate. The medium has the advantages of clear component, stability in quality, few impurities and the like, is suitable for industrial fermentation production and physiological research of Sparassis crispa and is favorable for separation and extraction of downstream metabolite.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a synthetic culture medium for Hydrangea broadleaf. Background technique [0002] Hydrangea broadleaf [Sparassis latifolia Y.C.Dai&Zheng Wang] is a rare edible and medicinal fungus, belonging to Kingdom Fungi (Kingdom Fungi), Basidiomycota (Basidiomycota), Polyporales (Polyporales), Hydrangeaceae in biological classification (Sparassidaceae), Hydrangea (Sparassis). Recent studies have shown that Hydrangea broadleaf is widely distributed in subtropical and temperate regions of East Asia, including Jilin, Heilongjiang, Yunnan, Japan and other places in China (Zhao et al., 2012, Mycological Progress, DOI 10.1007 / s11557-012-0853-7) , born in late summer and early autumn on the ground of spruce, fir or pine forests and mixed forests. [0003] Hydrangea is rich in protein, essential amino acids and vitamins and other nutrients (Chandrasekaran et al., 2011, International Journal of Medici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 董彩虹杨涛
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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