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Method for preparing xyio-oligosacchandes by using ultrasonic to treat agricultural wastes

A technology of agricultural waste and xylooligosaccharides, which is applied in the field of preparation of xylooligosaccharides, can solve the problems of high pollution, high cost, and long time

Inactive Publication Date: 2009-12-09
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xylanase can degrade xylans and exists widely in nature, and strains of xylanases that can degrade xylans, such as Trichoderma and Aspergillus, produce xylanases composed of Complex, often because of the highly active β-D-xylosidase, the content of xylooligosaccharides with a degree of polymerization of 2 to 5 in the product is not high, while the content of xylose is too high, which affects the quality and quality of xylooligosaccharides. Effect
[0012] The object of the present invention is exactly a kind of high efficiency specially developed for the above-mentioned problem, and the method for preparing xylo-oligosaccharides by ultrasonic treatment of agricultural wastes with low pollution, this method has overcome the high pollution, high Shortcomings such as cost and long time, and the application of special xylanase, on the one hand reduces the amount of xylanase and reduces the cost of raw materials; on the other hand, the products are mainly xylobiose and xylotriose, which are relatively pure and have a high yield , compared with the current method of producing xylooligosaccharides, the content of the target product is high, which reduces the difficulty of refining subsequent products and reduces production costs, so it has a good development prospect

Method used

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  • Method for preparing xyio-oligosacchandes by using ultrasonic to treat agricultural wastes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: Disperse the corn cob powder particles in a 10% (w / v) NaOH solution at a solid-to-liquid ratio of 1:10, and ultrasonically treat them for 30-40 minutes at 45-60°C and 110-130W; the optimum is 50 ℃, ultrasonic treatment at 120W for 35min;

[0029] Step 2: filtering to remove solids, collecting the filtrate, and adjusting the pH value to slightly acidic with an acidic buffer to obtain a hemicellulose solution;

[0030] Step 3: Add purified Streptomyces rameus endo-xylanase to the above hemicellulose solution to hydrolyze hemicellulose, wherein the amount of enzyme added is 50U / g substrate, and the reaction temperature is 45- 55°C, preferably 50°C, constant temperature shaking enzymatic hydrolysis for 24 hours;

[0031] Step 4: Boil the above solution in boiling water for 10 minutes to inactivate enzymes, centrifuge, separate, collect the supernatant, and then refine through processes such as decolorization, ion exchange, and concentration to obtain xylooligosacch...

Embodiment 2

[0033] Step 1: Disperse the bean stalk powder particles in a 10% (w / v) NaOH solution at a solid-to-liquid ratio of 1:10, and ultrasonically treat them for 30-40 minutes at 60-80°C and 180-200W; the optimum is 70 ℃, ultrasonic treatment at 190W for 35min;

[0034] Step 2: filtering to remove solids, collecting the filtrate, and adjusting the pH value to slightly acidic with an acidic buffer to obtain a hemicellulose solution;

[0035] Step 3: Add purified Streptomyces rameus endo-xylanase to the above hemicellulose solution to hydrolyze hemicellulose, wherein the amount of enzyme added is 50U / g substrate, and the reaction temperature is 45- 55°C, preferably 50°C, constant temperature shaking enzymatic hydrolysis for 24 hours;

[0036] Step 4: Boil the above solution in boiling water for 10 minutes to inactivate enzymes, centrifuge, separate, collect the supernatant, and then refine through processes such as decolorization, ion exchange, and concentration to obtain xylooligosac...

Embodiment 3

[0038] Step 1: Disperse the cottonseed hull powder particles in a 10% (w / v) NaOH solution at a solid-to-liquid ratio of 1:10, and ultrasonically treat them for 30-40 minutes at 60-80°C and 180-200W; the optimum is 70 ℃, ultrasonic treatment at 190W for 35min;

[0039] Step 2: filtering to remove solids, collecting the filtrate, and adjusting the pH value to slightly acidic with an acidic buffer to obtain a hemicellulose solution;

[0040] Step 3: adding purified Streptomyces rameus endo-xylanase to the above hemicellulose solution to hydrolyze hemicellulose, wherein the amount of enzyme added is 50U / g substrate, and the reaction temperature is 4555°C , the optimum temperature is 50°C, constant temperature shaking enzymatic hydrolysis for 24 hours;

[0041] Step 4: Boil the above solution in boiling water for 10 minutes to inactivate enzymes, centrifuge, separate, collect the supernatant, and then refine through processes such as decolorization, ion exchange, and concentration...

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PUM

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Abstract

The invention discloses a method for preparing functional xyio-oligosacchandes by taking agricultural wastes as raw materials and using ultrasonic treatment and bio-enzyme degradation. The method comprises the following steps: hydrolyzing the agricultural wastes after the ultrasonic treatment by using endo xylanase generated by Streptomyces rameus, converting hemicellulose in the agricultural wastes into the high-quality xyio-oligosacchandes, and preparing a xyio-oligosacchandes product having low xylose content and the polymerization degree between 2 and 5.

Description

technical field [0001] The invention relates to a method for preparing xylooligosaccharides, which uses ultrasonic treatment and biological enzyme degradation of agricultural wastes (such as corncobs, straws, rice husks, bean stalks, bagasse, etc.) to produce xylooligosaccharides. Background technique [0002] Xylooligosaccharides (also known as xylooligosaccharides) are 2-7 glycosidic bonds formed by endoxylanase hydrolyzing xylan with xylan as a substrate. A small molecular oligosaccharide mixture of D-xylose, the main components of which are xylobiose and xylotriose. At present, studies have confirmed that the main physiological functions of xylooligosaccharides include the following aspects: [0003] (1) Provide low energy, meet the requirements of those who like desserts but are worried about gaining weight, and can also be eaten by diabetics, obese patients and hypertensive patients; [0004] (2) Activate bifidobacteria in the intestinal tract and promote their proli...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/00A23L1/09A23L29/30A23L33/125
Inventor 李秀婷吕跃钢曹雁平马家津宋焕禄
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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