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Full-automatic serial fluorescence PCR detection system

A detection system, fully automatic technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems affecting the accuracy of detection results, achieve maximum flexibility and applicability, and reduce impact , the effect of improving reliability

Inactive Publication Date: 2009-12-30
GUANGZHOU INST OF RESPIRATORY DISEASE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the entire PCR detection process, many human factors are incorporated, which affect the accuracy of the detection results

Method used

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  • Full-automatic serial fluorescence PCR detection system
  • Full-automatic serial fluorescence PCR detection system
  • Full-automatic serial fluorescence PCR detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] like figure 1 As shown, it is a three-dimensional schematic diagram of a preferred embodiment of the sample device to be tested in the fully automatic serial fluorescent PCR detection system of the present invention. As can be seen from the figure, the sample device to be tested includes a sample disc 13, a rotating shaft 12 and a stepping The motor 11 and the sample disc 13 are connected to the stepping motor 11 through the rotating shaft 12. The sample disc 13 is regularly distributed with a number of sample holes 130 for inserting the sample tubes 15 along the circumference of the disc surface. The worker puts the sample to be tested into the sample tube 15 and then inserts it into the sample hole 130 . At the same time, a barcode is pasted on the tube side of the sample tube 15, and the photoelectric reader 14 distinguishes and tracks and memorizes the reaction state of each sample tube to be tested by identifying the barcode on the sample tube 15. In this embodime...

Embodiment 2

[0033] like figure 2Shown is a top view of a preferred embodiment of the nucleic acid processing device in the fully automatic serial fluorescent PCR detection system of the present invention. The nucleic acid processing device comprises a nucleic acid processing disc 21, a rotary mechanical push rod 23, a nucleic acid processor 24, and may also include a rotary head changer 22; the rotary mechanical push rod 23 and the rotary head changer 22 move at the center of the nucleic acid processing disc 21 Connection; the nucleic acid processing disc 21 includes an inner ring and an outer ring, forty-eight storage positions 211 and twenty-four nucleic acid processing positions 212 are set along the circumference of the disc, the storage positions 211 are in the inner ring, and the nucleic acid processing positions 212 are in the outer ring; the outer ring is fixed , the inner ring is controlled by a computer to rotate. The nucleic acid processor is located on the periphery of the n...

Embodiment 3

[0035] like image 3 Shown is a schematic diagram of a preferred embodiment of the 48°C reaction tray in the fully automatic serial fluorescent PCR detection system of the present invention.

[0036] Figure 3-1 It is a schematic side view of the 48°C reaction disk in this embodiment, which shows that the 48°C reaction disk includes a constant temperature rotating disk 31, a cover plate 32 and a fixed shaft 33, the cover plate 32 is connected with the fixed shaft 33, and the fixed shaft 33 is located at 48 The center of the ℃ reaction plate; the 48 ℃ reaction plate is located under the cover plate 32, and the constant temperature rotating plate 31 has a hole 311 for holding the reaction cup; the side of the cover plate 32 has a T-shaped slot opening card 321; T-shaped slot opening card 321 It corresponds to the hole 311 on the 48°C reaction plate.

[0037] Figure 3-2 It is a schematic top view of the 48°C reaction plate in this embodiment, which shows that the constant tem...

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PUM

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Abstract

The invention discloses a full-automatic serial fluorescence PCR detection system, comprising a photoelectric reader, a sample device to be detected, a nucleic acid processing unit, a reaction unit, a manipulator and a guide rail; wherein the photoelectric reader is connected with the sample device to be detected, the guide rail and the manipulator are arranged between the sample device to be detected and the nucleic acid processing unit, and the manipulator is arranged between the nucleic acid processing unit and the reaction unit. The full-automatic serial fluorescence PCR detection system of the invention is a device which is variable in temperature and acquires fluorescence information designed for realizing full-automatic PCR reaction process, innovatively improves the traditional PCR amplifier and changes the batch reaction mode of the traditional PCR amplifier, flow line PCR operating mode is provided, utilization rate of PCR devices is improved, full automation without human intervention of the whole detection process is realized, labour force is saved, working efficiency is improved, and meanwhile influence caused by human factor is also reduced.

Description

technical field [0001] The invention relates to a gene detection instrument used in the fields of biology, medicine, etc., especially a serial fluorescent PCR detection system capable of realizing the automation of the whole process from sample processing to reaction completion. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction), referred to as PCR, has become a routine technique for nucleic acid detection. In a thermal cycler, a thermal cycler block has one or more small holes for holding small tubes containing the reactant mixture. The temperature of the cycle block is cycled between a relatively low-temperature annealing stage of about 50-55 degrees and a high-temperature denaturation stage of about 90-95 degrees, and the polymerase chain extension reaction is completed during the heating process. The reaction sample goes through repeated "denaturation", "annealing" and "extension" stages on the thermal cycle block, that is, it is treated...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/34
Inventor 周荣苏晓波郭绍华
Owner GUANGZHOU INST OF RESPIRATORY DISEASE
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