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Magnetic particle Chemiluminescence Immunoassay determination kit of luteinizing hormone

A technology for luteinizing hormone production and chemiluminescence, which is applied in the field of magnetic particle chemiluminescence immunoassay assay kit and its preparation, can solve the problems of needs, limited popularization, and inability to be widely used in clinical diagnosis and scientific research, and achieves convenience in use , no radioactive pollution, simple and reliable sample pretreatment process

Inactive Publication Date: 2009-12-30
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemiluminescence immunoassay kit in the prior art is a closed fully automatic chemiluminescence measurement system, which requires an expensive fully automatic chemiluminescence measuring instrument, thus limiting its popularization and use, and cannot be widely used in clinical diagnosis and scientific research

Method used

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  • Magnetic particle Chemiluminescence Immunoassay determination kit of luteinizing hormone
  • Magnetic particle Chemiluminescence Immunoassay determination kit of luteinizing hormone
  • Magnetic particle Chemiluminescence Immunoassay determination kit of luteinizing hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Preparation I of the LH magnetic particle chemiluminescence immunoassay assay kit of the present invention

[0047] 1. Preparation of LH series calibrator

[0048] Dilute the pure LH product with horse serum into a calibrator, and distribute it into 7 bottles with concentrations of 0, 0.5, 2, 10, 50, 100, and 200mIU / mL.

[0049] 2. Preparation of magnetic particle solution coated with anti-FITC monoclonal antibody

[0050] Activate the magnetic particles with a particle size of 2.8 μm with glutaraldehyde, stir at room temperature, mix for 2 hours, apply a magnetic field, the magnetic field strength is 2000 gauss, let stand for 25 minutes, pour out the supernatant, and use 0.01 pH value of 7.4 Wash 3 times with mol / L phosphate buffer, and suspend with the buffer at a concentration of 100 mg / mL; add 100 μg of anti-FITC monoclonal antibody to each ml of suspension, stir overnight at 4°C, and then apply a magnetic field to increase the strength of the magnetic...

Embodiment 2

[0070] Embodiment 2 Preparation II of the LH magnetic particle chemiluminescence immunoassay assay kit of the present invention

[0071] 1. The preparation of LH series calibrator is the same as in Example 1;

[0072] 2. Preparation of magnetic particle solution coated with anti-FITC monoclonal antibody

[0073] Activate the magnetic particles with a particle size of 2.0 μm with glutaraldehyde, stir at room temperature, mix for 2 hours, apply a magnetic field, the magnetic field strength is 2000 gauss, let stand for 25 minutes, pour out the supernatant, and use 0.01 pH value of 7.4 mol / L phosphate buffer solution was washed 4 times, and the solution was used to suspend at a concentration of 50 mg / mL; 80 μg of anti-FITC monoclonal antibody was added to each ml of the suspension, and after stirring overnight at 4°C, a magnetic field was added, and the magnetic field strength was 2000 gauss, let stand for 15min, pour out the supernatant, and block with phosphate buffer (pH 7.2) ...

Embodiment 3

[0091] Embodiment 3 Preparation III of the LH magnetic particle chemiluminescence immunoassay assay kit of the present invention

[0092] 1. The preparation of LH series calibrator is the same as in Example 1;

[0093] 2. Preparation of magnetic particle solution coated with anti-FITC monoclonal antibody

[0094] Activate the magnetic particles with a particle size of 3.0 μm with glutaraldehyde, stir at room temperature, mix for 2 hours, apply a magnetic field, the magnetic field strength is 2000 gauss, let stand for 20 minutes, pour out the supernatant, and use 0.01 pH value of 7.4 Wash three times with mol / L phosphate buffer solution, and use this solution to suspend at a concentration of 75 mg / mL; add 60 μg of anti-FITC monoclonal antibody to each ml of suspension, stir overnight at 4°C, and then apply a magnetic field with a magnetic field strength of 2000 Gaussian, let stand for 10min, pour out the supernatant, and block with phosphate buffer (pH 7.2) containing 0.5% bov...

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Abstract

The invention discloses a magnetic particle Chemiluminescence Immunoassay determination kit of luteinizing hormone (LH), belonging to the field of immunoassay medical science. The kit comprises: 1) LH series of calibrators; (2) magnetic particle solution coated by monoclonal antibody resisting fluorescein isothiocyanate; (3) LH monoclonal antibody labeled by fluorescein isothiocyanate; (4) LH monoclonal antibody labeled by horse radish peroxidase; (5) chemiluminescence substrate liquid; and (6) washing concentrate. The invention also discloses a preparation method of the kit, and the kit has the advantages of simpleness, convenience, rapidness, sensitivity, stability, etc.

Description

technical field [0001] The invention belongs to the field of immunoanalysis medicine, and in particular relates to a magnetic particle chemiluminescence immunoassay assay kit for luteinizing hormone and a preparation method thereof. The kit of the invention combines immunomagnetic particle separation technology and chemiluminescence immunoassay technology. Background technique [0002] Luteinizing hormone (LH) is a reproductive hormone secreted by the pituitary gland. It stimulates the secretion of gonadotropin-releasing hormone (Gn-RH) by the hypothalamus. It has two polypeptide chains through the non- Covalently bound glycoprotein hormones. Its molecular weight is about 30kDa. The production of LH is controlled by the hypothalamic gonadotropin-releasing hormone, and at the same time, it is regulated by the positive and negative feedback of the gonads. In women, LH promotes the development and maturation of follicles, induces ovulation, promotes corpus luteum production,...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/74G01N33/535G01N21/76
Inventor 林金明肖勤
Owner TSINGHUA UNIV
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