Astragalus polysaccharide extraction method

An astragalus polysaccharide and an extraction method technology are applied in the field of extracting astragalus polysaccharide from traditional Chinese medicine Astragalus, which can solve the problems of low extraction rate, large loss of effective components, difficult large-scale application, etc. Easier dissolution effect

Inactive Publication Date: 2010-01-06
HANGZHOU KEHUANG ENTERPRISE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate of water extraction method is low, and the steps are complicated, time-consuming, and the loss of active ingredients is large; the microwave method has high requirements for equipment, is difficult to implement, and is difficult to apply on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 70g of astragalus root powder and place it in a 500ml three-neck flask, add 140ml of ethanol with a concentration of 70% dropwise to make the material evenly wet, and let it stand for 30min.

[0025] Add 245ml of distilled water at a temperature of 100°C to the above materials, keep warm with a thermostat, reflux for 20 minutes, cool, and filter; the filter cake is washed repeatedly with hot water at 40°C, mixed with the filtrate and cleaning solution, and the filter cake is set aside.

[0026] Add 5 times the weight of water to the above filter cake, add 0.35 g of neutral cellulase, control the temperature at 48° C., adjust the pH to neutral, and perform enzymatic hydrolysis for 2 hours. After enzymolysis, heat the material to 80°C, inactivate the enzyme, cool, filter, and wash, combine the filtrate and cleaning solution, and discard the filter cake.

[0027] Combine the filtrate and cleaning solution obtained in the above steps, concentrate under reduced pressur...

Embodiment 2

[0029] Weigh 50g of astragalus root powder and put it in a 500ml three-neck flask, add 75ml of ethanol with a concentration of 95% dropwise to make the material evenly wet, and let it stand for 30min.

[0030] Add 250ml of distilled water at a temperature of 100°C to the above materials, keep warm with a thermostat, reflux for 20 minutes, cool, and filter; the filter cake is washed repeatedly with hot water at 40°C, mixed with the filtrate and cleaning solution, and the filter cake is set aside.

[0031] Add 10 times the weight of water to the above filter cake, add 0.5 g of neutral cellulase, control the temperature at 50° C., adjust the pH to be neutral, and perform enzymatic hydrolysis for 5 hours. After enzymolysis, heat the material to 80°C, inactivate the enzyme, cool, filter, and wash, combine the filtrate and cleaning solution, and discard the filter cake. Combine the filtrate and cleaning solution obtained in the above steps, concentrate under reduced pressure to 20% ...

Embodiment 3

[0033] Weigh 65g of astragalus root powder and place it in a 500ml three-neck flask, add 140ml of ethanol with a concentration of 70% dropwise to make the material evenly wet, and let it stand for 30min.

[0034] Add 200ml of distilled water at a temperature of 90°C to the above materials, keep warm with a thermostat, reflux for 20 minutes, cool, and filter; the filter cake is washed repeatedly with hot water at 40°C, mixed with the filtrate and cleaning solution, and the filter cake is set aside.

[0035] Add 5 times the weight of water to the above filter cake, add 0.46 g of neutral cellulase, control the temperature at 48° C., adjust the pH to neutral, and perform enzymatic hydrolysis for 2 hours. After enzymolysis, heat the material to 80°C, inactivate the enzyme, cool, filter, and wash, combine the filtrate and cleaning solution, and discard the filter cake. Combine the filtrate and cleaning solution obtained in the above steps, concentrate under reduced pressure to 20% o...

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PUM

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Abstract

The invention discloses an astragalus polysaccharide extraction method, comprising the following steps: adding ethanol to soak astragalus root powder; adding water to carry out thermal extraction; carrying out the enzymolysis of filter cakes and enzyme destruction; concentrating extract, depositing the extract with ethanol, drying the extract and the like. Compared with the traditional method, by using ethanol solution as strippant, taking water as extraction solvent, and adopting desorbing-internal boiling method, the method provided by the invention has significant improvement in extraction speed and extraction ratio, keeps the activity of the astragalus polysaccharide, and has the advantages of short time consumption, high extraction rate, less impurity and low energy consumption in the extraction process, therefore, the method is a green, safe and efficient . The method is also suitable to the extraction and the preparation of other plant-derived medicine polysaccharide.

Description

technical field [0001] The invention relates to a method for extracting plant polysaccharides, in particular to a method for extracting astragalus polysaccharides from traditional Chinese medicine astragalus. Background technique [0002] With the large-scale breeding of livestock and poultry, their stocking density continues to increase. In order to reduce the incidence of livestock and poultry diseases, prevent and control the occurrence of diseases, the use of a large number of antibiotics and antiviral drugs in the breeding industry has caused the drug residues in livestock and poultry products to exceed the standard. The continuous growth of drug-resistant pathogenic microorganisms has led to the continuous decline of animal immunity, which has caused huge losses to the animal husbandry. [0003] At present, immunotherapy, a safe and green treatment method, has become the common goal of people in the industry all over the world. Because many Chinese herbal medicines h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 陈剑英傅锦浩
Owner HANGZHOU KEHUANG ENTERPRISE
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