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Method for preparing flavonoid compound by guava cell culture method

A technology of flavonoids and cell culture, applied in the direction of fermentation, can solve the problem of not meeting the needs of the market, and achieve the effects of improving resource utilization, saving farmland, and high efficiency

Inactive Publication Date: 2010-01-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the current market demand for flavonoids is growing rapidly, and the current production method cannot meet the market demand

Method used

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Experimental program
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Embodiment Construction

[0032] Now take the laboratory culture as an example, and the non-limiting examples are described as follows:

[0033] Describe in detail the technological process (not limited to this embodiment) that pomegranate cell culture of the present invention produces flavonoids with embodiment form below:

[0034] 1. Preparation and sterilization of culture medium

[0035] In laboratory culture, prepare MS growth medium, agar 7g / L, sucrose concentration 30g / L, adjust the pH value of the medium at 5.6. Then divide into 100ml Erlenmeyer flasks, 20-30ml per bottle. After sealing with cotton and paper, perform high-pressure disinfection at a pressure of 1.0-1.2Kg / cm 2 , the time is 20-25 minutes. The pomegranate seeds were sterilized in 70% alcohol and 0.1% mercuric chloride, then inserted into the above-mentioned medium, and cultivated under light at 25±1° C. for 10 days to obtain aseptic pomegranate seedlings.

[0036] 2. Callus culture and subculture optimization

[0037] Prepare...

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Abstract

The invention relates to a method for preparing a flavonoid compound by a guava cell culture method, which comprises the following steps: firstly, preparing aseptic seedlings by using guava seeds; inoculating the aseptic seedlings on a solid substrate (I) by using the aseptic seedlings as explants; culturing callus at 24-26 DEG C; inoculating the callus in a liquid substrate (II) to carry out seed culture and production culture; separating and purifying to obtain general flavone. The substrate (I) is formed by adding 0.5-2.0 mg / L of 2,-4D and 0.0-0.5mg / L of KT, and a pH value is from 5.5 to 5.7; the substrate (II) is an MS substrate without agaragar, and a pH value is from 5.4 to 5.6; the total flavone content in a culture reaches 8.31 percent and is further higher than the flavone content in guava.

Description

1. Technical field [0001] The invention relates to a method for preparing biopharmaceuticals by a fine suspension cell culture method, in particular to a method for producing flavonoids by using pomegranate callus for cell suspension culture. 2. Background technology [0002] Humans have a long history of utilizing plant secondary metabolites, and the research on plant secondary metabolites is in full swing today. However, the yield of plant secondary metabolites is limited by plant yield and secondary biomass content, and some secondary metabolites have very little content in nature, which limits the wide application of plant secondary metabolites. With the great progress made in biotechnology, people focus on the research on how to use biotechnology to obtain plant secondary matter. For this reason, relevant research scientists have begun unremitting research and efforts. After decades of exploration, it has been discovered that the "factory" efficient production of plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 丁之恩程江华
Owner ANHUI AGRICULTURAL UNIVERSITY