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Method for detecting air quality of hospital by utilizing chicken blood nutrient agar panel

A technology for nutrient agar and air quality, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve the problems of difficulty in detection of nutrient agar plates, difficulties in blood collection of raw materials for blood nutrient agar plates, etc.

Inactive Publication Date: 2010-01-06
郭丽芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method for detecting air quality in hospitals with chicken blood nutrient agar plates in order to overcome the existing nutrient agar plate detection difficulties and blood nutrient agar plate raw blood collection difficulties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The preparation of embodiment 1 chicken blood nutrient agar plate

[0011] Pour the sterilized and melted ordinary nutrient agar into a flat plate (φ90mm) when it is cooled to 45°C, add 10ml of defibrated cotton chicken blood per 100ml when it is cooled to 45°C, mix well and then pour into a flat plate, and the chicken blood nutrition can be obtained after cooling Agar plates.

Embodiment 2

[0012] Example 2 Detect total number of bacteria and number of pathogenic bacteria in hospital air

[0013] Select different sampling points according to the indoor area, put the chicken blood nutrient agar plate at the above points at the same time, open the cover of the plate and place it next to the plate, cover the plate after 5 minutes of exposure, wrap it with sterilized paper and send it for inspection immediately.

[0014] Put the sample sent for inspection after sampling in an incubator at 36±1°C for 48 hours, count and record the number of colonies on each plate of the sample, and carry out detection and identification of pathogenic bacteria at the same time. The total number of bacteria was no different from that on the ordinary nutrient agar plate (P>0.05), and the detection result of pathogenic bacteria was better than that on the ordinary nutrient agar plate (P<0.05).

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Abstract

The invention relates to a method for detecting the air quality of a hospital by utilizing a chicken blood nutrient agar panel. Specifically, chicken blood is utilized to manufacture a panel so as to obtain a novel blood nutrient agar panel, and the blood nutrient agar panel is utilized to detect the total number of bacteria and the number of pathogenic bacteria. The invention belongs to the field of environmental detection.

Description

technical field [0001] The invention relates to a method for detecting air quality in a hospital by using chicken blood nutrient agar plate. Specifically, chicken blood is used to make plates to obtain a new type of blood nutrient agar plate, and it is used to detect the total number of bacteria and pathogenic bacteria in the hospital air. This research belongs to the field of environmental detection. Background technique [0002] The quality of hospital environment is an important index to control the occurrence of nosocomial infection. According to the "Technical Specifications for Disinfection", when monitoring the air in Class I, II, and III areas of hospitals, nutrient agar plates are used for sample collection and testing. For the detection of staphylococcus aureus, hemolytic streptococcus and other pathogenic bacteria, it is necessary to identify colonies one by one. When the sample volume is large or there are many colonies growing, it is inevitable to miss it. If ...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/04
Inventor 郭丽芳
Owner 郭丽芳
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