Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress
A salt stress, cucumber technology, applied in the field of promoting cucumber seed germination under salt stress conditions, can solve the problems of ion poisoning, reduce seed germination rate and the like, and achieve the effects of improving utilization rate and improving germination rate
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[0010] Example 1,
[0011] The present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments. All putrescine used in the present invention was purchased from sigma company.
[0012] Example 1
[0013] The cucumber variety selected for the experiment was Zhongnong No. 8, from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, wash them with distilled water, and soak the above-mentioned cucumber seeds with the following seed soaking solutions a, b and c, respectively, for a soaking time of 24 hours and a seed soaking temperature of 20°C.
[0014] Seed soaking solutions a, b and c are:
[0015] a. Water;
[0016] b. CaCl at a concentration of 0.5mM 2 Aqueous solution
[0017] c, L-glutamic acid and CaCl 2 The aqueous solution; Among them, the concentration of L-glutamic acid is 5mM, CaCl 2 The c...
Example Embodiment
[0021] Example 2
[0022] The cucumber variety selected for the experiment was Zhongnong No. 8, from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, wash them with distilled water, and soak the above-mentioned cucumber seeds with the following seed soaking solutions a, b, and c, respectively, for a soaking time of 12 hours and a seed soaking temperature of 28°C.
[0023] Seed soaking solutions a, b and c are:
[0024] a. Water;
[0025] b. CaCl at a concentration of 0.5mM 2 Aqueous solution
[0026] c, L-glutamic acid and CaCl 2 The aqueous solution; Among them, the concentration of L-glutamic acid is 10mM, CaCl 2 The concentration is 0.5mM.
[0027] The above-mentioned seed soaking solutions a, b, and c are each set in 4 repetitions, and 5ml of seed soaking liquid is added to each repetition.
[0028] After the seed soaking is completed, put two layers of filter paper in a petri dish wit...
Example Embodiment
[0030] Example 3
[0031] The cucumber variety selected for the experiment was Zhongnong No. 8, from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, wash them with distilled water, and soak the above-mentioned cucumber seeds with the following seed soaking solutions a, b and c, respectively, for a soaking time of 18 hours and a seed soaking temperature of 25°C.
[0032] Seed soaking solutions a, b and c are:
[0033] a. Water;
[0034] b. CaCl at a concentration of 0.5mM 2 Aqueous solution
[0035] c, L-glutamic acid and CaCl 2 The aqueous solution; Among them, the concentration of L-glutamic acid is 20mM, CaCl 2 The concentration is 0.5mM.
[0036] The above-mentioned seed soaking solutions a, b, and c are each set in 4 repetitions, and 5 ml of seed soaking liquid is added to each repetition.
[0037] After the seed soaking is completed, put two layers of filter paper in a petri dish wit...
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