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Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress

A salt stress, cucumber technology, applied in the field of promoting cucumber seed germination under salt stress conditions, can solve the problems of ion poisoning, reduce seed germination rate and the like, and achieve the effects of improving utilization rate and improving germination rate

Inactive Publication Date: 2010-01-13
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under the condition of salt stress, water and ion uptake in plant cells are changed, resulting in hyperosmotic stress, ion poisoning, thereby reducing the germination rate of seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to the following examples. Putrescine used in the present invention was purchased from sigma company.

[0012] Example 1

[0013] The cucumber variety used in the experiment is Zhongnong No. 8, which comes from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, and wash them with distilled water. Soak the cucumber seeds in the following soaking solutions a, b, and c respectively. The soaking time is 24 hours, and the soaking temperature is 20°C.

[0014] Seed soaking solutions a, b and c are respectively:

[0015] a. water;

[0016] b. CaCl with a concentration of 0.5mM 2 aqueous solution;

[0017] c, L-glutamic acid and CaCl 2 aqueous solution; wherein, the concentration of L-glutamic acid is 5mM, CaCl 2 The concentration is 0.5mM.

...

Embodiment 2

[0022] The cucumber variety used in the experiment is Zhongnong No. 8, which comes from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, and wash them with distilled water. Soak the cucumber seeds in the following soaking solutions a, b, and c respectively. The soaking time is 12 hours, and the soaking temperature is 28°C.

[0023] Seed soaking solutions a, b and c are respectively:

[0024] a. water;

[0025] b. CaCl with a concentration of 0.5mM 2 aqueous solution;

[0026] c, L-glutamic acid and CaCl 2 aqueous solution; wherein, the concentration of L-glutamic acid is 10mM, CaCl 2 The concentration is 0.5mM.

[0027] The above seed soaking liquids a, b and c were respectively set to 4 repetitions, and 5ml of seed soaking liquid was added to each repetition.

[0028] After seed soaking is completed, put 2 layers of filter paper in a petri dish with a diameter of 9 cm. Af...

Embodiment 3

[0031] The cucumber variety used in the experiment is Zhongnong No. 8, which comes from the Chinese Academy of Agricultural Sciences. Take the plump and consistent Zhongnong No. 8 cucumber seeds, disinfect them with sodium hypochlorite, and wash them with distilled water. Soak the cucumber seeds in the following soaking solutions a, b, and c respectively. The soaking time is 18 hours, and the soaking temperature is 25°C.

[0032] Seed soaking solutions a, b and c are respectively:

[0033] a. water;

[0034] b. CaCl with a concentration of 0.5mM 2 aqueous solution;

[0035] c, L-glutamic acid and CaCl 2 aqueous solution; wherein, the concentration of L-glutamic acid is 20mM, CaCl 2 The concentration is 0.5mM.

[0036] The above seed soaking liquids a, b and c were respectively set to 4 repetitions, and 5ml of seed soaking liquid was added to each repetition.

[0037] After seed soaking is completed, put 2 layers of filter paper in a petri dish with a diameter of 9 cm. Af...

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Abstract

The invention discloses a method and a special seed soaking agent for promoting germination of cucumber seeds under the condition of salt stress. The seed soaking agent comprises calcium chloride, L-glutamic acid and water, wherein the molar concentration ratio of the calcium chloride to the L-glutamic acid is 0.5:5-20, preferably 0.5:10. The method for promoting germination of cucumber seeds under the condition of salt stress comprises the step that the seed soaking agent for promoting germination of cucumber seeds under the condition of salt stress is used to soak the cucumber seeds before seeding. The method for promoting germination of cucumber seeds under the condition of salt stress can obviously improve the germination ratio of the cucumber seeds under the condition of salt stress, and the germination ratio is more than 45%. The method is very favorable for improving the utilization ratio of resources and provides the guarantee for vegetable production in salinized areas.

Description

technical field [0001] The invention relates to a method for promoting germination of cucumber seeds under salt stress conditions and a special seed soaking solution. Background technique [0002] Due to environmental pollution, soil salinization has become a worldwide problem, and the area of ​​various saline-alkali lands in my country is also increasing (Yang Shaohui et al. Research progress on the impact of salt stress on plants [J]. Molecular Plant Breeding, 2006, 4 : 139-142). At present, there is no uniform standard for the lower limit of salt stress concentration, and different plant species show different sensitivities to salt stress. It is generally believed that NaCl is the most harmful to plants among inorganic salts. Even a low salinity of 50mM NaCl is fatal to rice with strong salt tolerance (Lin Qifeng et al. Research progress on plant salt tolerance [J]. Bioengineering progress , 2005, 20:20-25). A large number of studies have proved that most vegetable plan...

Claims

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Application Information

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IPC IPC(8): A01N59/08A01N37/44A01P21/00A01C1/00
Inventor 张文浩李银心王宝兰常晨硕
Owner INST OF BOTANY CHINESE ACAD OF SCI
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