Method for breeding cordyceps militars strain

A technology of strain selection and Cordyceps militaris, applied in botany equipment and methods, fertilizer mixture, horticulture, etc., can solve the problems of low efficiency and achieve the effect of excellent character and high vitality

Inactive Publication Date: 2010-01-20
HESHAN ZHONGCHUN BIOTECH
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Problems solved by technology

This method can obtain pure strains, but because of the huge number of single spores, there are also many colonies formed by growth, and a large amount of screening work is still required, and some colonies with excellent properties are selected for subsequent cultivation. The workload has not been reduced much, and the efficiency is still not high

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Embodiment Construction

[0017] Below in conjunction with example, further illustrate the present invention.

[0018] A kind of Cordyceps militaris bacterial classification breeding method, comprises the following steps:

[0019] 1) Scrape the spores from the surface of the Cordyceps militaris moss, add them into a triangular flask containing sterile water and a dispersant of Cordyceps militaris spores Tween-80, add glass beads into the triangular flask, shake well with a rotary oscillator, Dilute to required multiple then, make Cordyceps militaris spore suspension;

[0020] 2) prepare the nutrient-deficient basal medium: PDA basal medium, its formula is:

[0021] Water: 600-800ml Potato: 80-100g Glucose: 15-18g Agar: 15-18g. 12-16ml concentration of bacteria is 2% sodium deoxycholate solution and 1.8-2ml concentration is 10000-15000u / ml streptomycin solution, mix evenly, make PDA basic selection medium;

[0022] 3) Prepare nutrient-rich enriched medium: enrich PDA medium, its formula is:

[0023]...

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Abstract

The invention discloses a method for breeding cordyceps militars strain, which comprises the steps of uniformly mixing the cordyceps militars spore suspension with a basic culture medium lack of nutrition, pouring a layer of basic culture medium after the mixture of the cordyceps militars spore suspension and the basic culture medium lack of nutrition is coagulated, and pouring a layer of nutritious culture medium on the second coagulated culture medium, then performing inverted culture, and selecting the bacterial colony of cordyceps militars monad with excellent characters in the inverted culture process. In the invention, a great number of cordyceps militars spores with inferior character and low activity are eliminated through the selection of the culture medium layer by layer, leaving the pure cordyceps militars with superior character and high activity; therefore, the screening workload is decreased greatly, the bacterial colony with superior character can be more conveniently selected in the subsequent experiments, and the screening efficiency is improved effectively.

Description

technical field [0001] The invention relates to a method for breeding Cordyceps militaris strains. Background technique [0002] When the existing Cordyceps militaris strains are selected and bred, after the spores are obtained, the spores are generally diluted to an appropriate degree by means of streaking and coating on a plate, and further selection is performed after colonies are formed. Although this breeding method is simple to operate, due to the limitations of the technology itself, it is difficult to obtain colonies formed by the growth of single spores, and the obtained colonies need to be further separated and selected. This method requires a lot of repetitive labor, the separation efficiency is low, and the selection time is long, which is difficult to meet the needs of production. [0003] Some spores are also partially diluted to a certain extent by the gradient dilution method, and then spread and cultivated in the medium to obtain colonies formed by single s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 吕尚廉郑杰辉项坤
Owner HESHAN ZHONGCHUN BIOTECH
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