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Shaanxi euonymus glycoside II extracted from Shaanxi euonymus and extraction technique thereof

An extraction process, dulcitol technology, which is applied in the field of Shaanxi dulcitol glycoside II extracted from Shaanxi Euonymus and its extraction process, can solve the problems that there are no public reports on active ingredients, and achieve convenient operation and preparation method Advanced, Reliable Effects

Active Publication Date: 2012-07-04
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Why does Euonymus shaanxi have insecticidal activity? There is no public report on how to extract its active ingredients

Method used

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  • Shaanxi euonymus glycoside II extracted from Shaanxi euonymus and extraction technique thereof
  • Shaanxi euonymus glycoside II extracted from Shaanxi euonymus and extraction technique thereof
  • Shaanxi euonymus glycoside II extracted from Shaanxi euonymus and extraction technique thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 10kg of fresh Euonymus shaanxi stems and leaves from Henan, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 18°C. The first time is 20 minutes, the second time is 15 minutes, and the extracts are combined. Recover the solvent under reduced pressure at 45°C to obtain 410g of dry powder, add water to disperse and dissolve the dry powder, centrifuge, put the supernatant on a macroporous adsorption resin column Diaion HP-20, wash with water until the eluent is colorless, and then wash with 10% methanol until elution The liquid was colorless, and 10% methanol eluent was obtained, and the solvent was recovered under reduced pressure at 45°C to obtain 15 g of dry powder. The dry powder was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent, which was dripped Developed on a thin-layer plate, sprayed with anisaldehyde-concentrated sulfuric acid color developer after devel...

Embodiment 2

[0021] Take 15 kg of fresh Euonymus shaanxi stems and leaves from Shaanxi, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 20°C, each time for 18 minutes. After the extracts are combined, the solvent is recovered under reduced pressure at 45°C , to get dry powder 605g, the dry powder is dispersed and dissolved in water, centrifuged, the supernatant is put on a macroporous adsorption resin column DiaionHP-20, washed with water until the eluent is colorless, then washed with 10% methanol until the eluent is colorless, and 10% The methanol eluent was recovered under reduced pressure at 45°C to obtain 23 g of dry powder. The dry powder was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent. Spray with anisaldehyde-concentrated sulfuric acid developer, at 105°C, the fraction with an RF value of 0.3 will first appear blue and then turn red. Collect this fraction to obtain componen...

Embodiment 3

[0023] Take 20kg of fresh Euonymus shaanxi stems and leaves from Shandong, and use a flash extractor to extract twice in 70% acetone aqueous solution at room temperature at 22°C. The first time is 15 minutes, the second time is 20 minutes, and the extracts are combined. Recover the solvent under reduced pressure at 45°C to obtain 825 g of dry powder, add water to disperse and dissolve the dry powder, centrifuge, put Diaion HP-20 on the macroporous adsorption resin on the supernatant, wash with water until the eluent is colorless, and then wash with 10% methanol until elution The liquid was colorless, and 10% methanol eluent was obtained, and the solvent was recovered under reduced pressure at 45° C. to obtain 32 g of dry powder. The dry powder was dissolved in water and applied to a gel Toyopearl HW-40 column, and eluted with water to obtain an eluent, which was dripped on Developed on a thin-layer plate, sprayed with anisaldehyde-concentrated sulfuric acid color developer afte...

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Abstract

The invention relates to a Shaanxi euonymus glycoside II extracted from Shaanxi euonymus and an extraction technique thereof. The technique comprises: stems and leaves of fresh Shaanxi euonymus are taken and then processed by histiocyte cataclase in acetone water solution at the room temperature; solvent is extracted, decompressed and recovered to obtain dry powder which is dissolved and centrifugated when being added with water; supernate passes through macroporous absorption resin column and then washed by water and 10% of methyl alcohol until eluent is colorless; after that, 10% of methyl alcohol eluent can be obtained, and solvent is decompressed at the low temperature and recovered to obtain dry powder which is then dissolved in gel column and eluted by water, so that eluent can be obtained; then, the obtained eluent is dripped onto a thin layer plate to be spread, and sprayed by anisaldehyde-concentrated sulfuric acid color development reagent, so as to show blue at first and then be changed into red fluid; the fluid sequentially passes through MCI Gel CHP-20 column, toyopearl HW-40 column, hydroxypropyl sephadex LH-20 column and silicagel column, so that colorless oily matter which is the Shaanxi euonymus glycoside II can be obtained. The Shaanxi euonymus glycoside II is a new compound, and the preparation method of the compound is convenient in operation, strong in reliability and great in economic and social benefits.

Description

1. Technical field [0001] The present invention relates to Shanxi dulcitol glycoside II extracted from Euonymus shaanxi and its extraction process. 2. Background technology [0002] Shaanxi euonymus (Euonymus schensianus Maxim.) is a plant of the genus Euonymus (Celastraceae) in the family Celastraceae, also known as golden silk hanging butterfly and golden butterfly. According to the "Xinhua Compendium of Materia Medica", most of the plants in this family have insecticidal activity, mainly concentrated in the genus Tripterygium genus, Euonymus genus, and Snakevine genus. Since the 1980s, Chinese scholars have conducted extensive research on the insecticidal activity of many plants in the family Euonymus, and found that Euonymus shaanxi has antifeedant and anesthetic effects on insect larvae. Widely distributed in the southern part of North China and the provinces of central China, mainly in the Funiu Mountains of Henan Province. Why does Euonymus shaanxi have insecticidal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/26C07H1/08
Inventor 郑晓珂冯卫生郭锦辉王小兰
Owner HENAN UNIV OF CHINESE MEDICINE