Detecting kit for five kinds of pathogenic vibrio in aquatic product and detecting method thereof

A pathogenic Vibrio and detection kit technology, which is applied in the detection of pathogenic Vibrio in aquatic products by using mPCR-DHPLC technology, the composition used for detection, and the field of kits, which can solve economic losses and affect the analysis of results , false positives and other problems, to achieve the effect of short inspection time, simple method, labor and financial saving
CN101633952AInactive Publication Date: 2010-01-27曹际娟 +4
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
曹际娟
Publication Date
2010-01-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detecting kit for five kinds of pathogenic vibrio in aquatic product and a detecting method thereof, which is used for food microbial contamination detection and monitoring. In the kit, 1 mL of detecting solution contains 10 mM of Tris.Cl, 50mM of KCl, 25mM of MgCl2, 2.5mM of dNTP, 5U / muL of Taq DNA polyase and five kinds of 10 mu M pathogenic vibrio primers. The kit and the detecting method of the invention can detect 5 kinds of pathogenic vibrio in aquatic products once; the detection lower limit is lower than 100cfu / mL, so that the method of the invention can detect when the number of each pathogenic vibrio is only 100 in each mL of bacteria solution. In addition, the method has shorter detection time than the traditional method, is more convenient, can save great labor and money and is suitable for quick detecting requirements.
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Description

technical field

[0001] The invention relates to a method for detecting pathogenic microorganisms in food, in particular to a method for detecting pathogenic Vibrio bacteria in aquatic products by using mPCR-DHPLC technology. It also relates to compositions used in the assay, ie kits. Background technique

[0002] With the improvement of living standards and the gradual opening up after my country's entry into the WTO, people's demand for aquatic products is increasing, and the import and export trade of aquatic products is also expanding. However, the monitoring and detection of pathogenic microorganisms, especially pathogenic Vibrio, in aquatic products has always been one of the important issues restricting the trade of aquatic products in my country. In 1998 and 2000, my country's cod fillets exported to France and aquatic products exported to Sweden were detected with Vibrio vulnificus and Vibrio mimicus, and their code names were cancelled. Since 2001, all aquatic pro...

Claims

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