Rapid propagation technology with open tissue culture for xanthoceras sorbifolia bunge

A tissue culture, open technology, applied in the field of plant tissue culture, can solve the problems of salinity, short drug effect period, sterilization plant tissue damage, etc., and achieve the effect of reducing the cost of tissue culture and simplifying the process of tissue culture.

Inactive Publication Date: 2010-02-10
北京金桐福绿色能源科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The first reason is that there is no antibiotic that is effective against all bacteria, and the drug effect period is short; the second is that some antibioti

Method used

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  • Rapid propagation technology with open tissue culture for xanthoceras sorbifolia bunge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] 1. Primary culture (explant induction, sterile system establishment)

[0011] In sunny days, take the semi-lignified stems of the sprouting strips of X. sorbifolium, cut off the leaves, wrap them back with plastic film, rinse them repeatedly with running water and washing powder, and trim the materials into 3--4cm stems, with 1-2 Put an axillary bud into a beaker, add washing powder, seal it with gauze, rinse it with running water for 1-2 hours, cut off the stem after washing, and soak it in a 4% statin solution. The culture medium is MS0, in which sugar is 30g / L, agar powder is 4g / L, and a certain concentration of statin is added instead of autoclaving. In the culture room, the daytime temperature is (26±2)°C, the nighttime temperature is (20±2)°C, and the light time is 16 hours a day. The light source is an incandescent fluorescent lamp. Seven days after inoculation, the axillary buds began to swell and germinate, and after 25 days, the axillary buds grew to 1-2 cm l...

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Abstract

The invention belongs to the plant tissue culture field, and relates to a rapid propagation technology with open tissue culture for xanthoceras sorbifolia bunge. In the technology, plant tissue culture technical steps for the xanthoceras sorbifolia bunge are provided, the propagation technology with open tissue culture for the xanthoceras sorbifolia bunge is improved by an open tissue culture technology, a simple open tissue culture technology for the xanthoceras sorbifolia bunge is created, a seriously sterile operating environment, high pressure sterilization and a super clean bench are notrequired, and plant tissue culture can be carried out in a natural and open bacteria environment by adopting a plastic cup instead of a tissue culture bottle, thus fundamentally simplifying tissue culture links and reducing tissue culture cost.

Description

1. Technical field [0001] The invention belongs to the field of plant tissue culture, and relates to an open tissue culture rapid propagation technology of sorbifolium sorbifolium. 2. Background technology [0002] Xanthoceras sorbifolia Bunge., also known as Papaya, Wendengge, and Sengdeng Maodao, belongs to Sapindaceae (Sapindaceae), Xanthoceras (Xanthoceras), is a single species, deciduous tree or shrub, originally Produced in northern my country, it is distributed in the north of Qinling Mountains and Zhunhe River, south of Inner Mongolia, from Liaoning in the east, to Qinghai in the west, to Henan and northern Jiangsu in the south. The comprehensive utilization value of X. sorbifolium is high, and it can grow everywhere except in saline-alkali land or waterlogged depressions. It is a pioneer tree species for greening barren hills; it has a well-developed root system and strong root sprouting ability, and is an excellent tree species for water conservation and sand contro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 孙仲序董凤亮马海渊
Owner 北京金桐福绿色能源科技有限公司
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