Method for rapidly extracting resveratrol from mulberry leaves

A technology of resveratrol and mulberry leaves, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., to achieve the effect of ensuring follow-up resources, increasing added value, and getting rid of dependence

Inactive Publication Date: 2010-02-17
LUXI XIAOYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Crush and dry 100kg of mulberry leaves, add 300L of 70% ethanol, heat and reflux at 80°C for 2h, filter twice with three layers of gauze, filter with a ceramic membrane, concentrate under reduced pressure to 50L (no ethanol smell), and then use 50L of ethyl acetate to extract the concentrate Extraction, take the upper layer (ethyl acetate layer), and then extract the lower layer solution with 50L ethyl acetate, take the upper layer, and then extract the lower layer solution with 50L ethyl acetate, combine the three ethyl acetate extracts for a total of about 150L, ​​and use rotary evaporation Concentrate to dryness, then heat to dissolve with 80L of 70% ethanol, add 5kg of granular activated carbon for decolorization for 30min, then concentrate to dryness, heat to dissolve in 2L of 90% ethanol, then add hot water to mix until the final concentration of ethanol is 50%. Then crystallize at room temperature for 24 hours to obtain a resveratrol sample.

[0025] After testin...

Embodiment 2

[0027] Crush and dry 500kg of mulberry leaves, add 600L of 70% ethanol, heat and reflux at 80°C for 2h, filter twice with three layers of gauze, filter with a ceramic membrane, concentrate under reduced pressure to 100L (no ethanol smell), and then use 100L of ethyl acetate to extract the concentrate Extraction, take the upper layer (ethyl acetate layer), extract the lower layer solution with 100L ethyl acetate, take the upper layer, and extract the lower layer solution with 100L ethyl acetate, combine the three ethyl acetate extracts for a total of about 300L, and use rotary evaporation Concentrate to dryness, then heat to dissolve with 150L of 70% ethanol, add 15kg of activated carbon to decolorize for 30min, then concentrate to dryness, heat to dissolve in 6L of 90% ethanol, add hot water until the final concentration of ethanol is 47%, crystallize at room temperature for 24h, Obtain a resveratrol sample. Purity testing was carried out according to the method of Example 1, ...

Embodiment 3

[0029] Crush and dry 200kg of mulberry leaves, add 200L of 70% ethanol, heat and reflux at 80°C for 2h, filter twice with three layers of gauze, filter with a ceramic membrane, concentrate under reduced pressure to 50L (no ethanol smell), and then use 50L of ethyl acetate to extract the concentrated solution Extraction, take the upper layer (ethyl acetate layer), and then extract the lower layer solution with 50L ethyl acetate, take the upper layer, and then extract the lower layer solution with 50L ethyl acetate, combine the three ethyl acetate extracts for a total of about 150L, ​​and use rotary evaporation Concentrate to dryness, then heat to dissolve with 100L of 70% ethanol, add 8kg of activated carbon to decolorize for 30min, then concentrate to dryness, heat to dissolve in 3L of 90% ethanol, add hot water until the final concentration of ethanol is 40%, crystallize at room temperature for 24h, Obtain a resveratrol sample. Purity detection was carried out according to th...

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Abstract

The invention provides a method for rapidly extracting resveratrol from mulberry leaves, which greatly improves the yield and the purity of the resveratrol. The invention provides a set of proceduresfor extracting and separating high-purity resveratrol from the mulberry leaves, and the pure resveratrol is obtained by the following procedures: backflow extraction by adopting alcohol, membrane filtration, concentration, extraction, decoloration by active carbon, and alcohol crystallization and recrystallization. The integral separation process has low requirement on environment conditions, theseparation time is shortest and the purity is highest. Separating materials are easy to obtain and cheap, the separation operation process is simple and easy to control, the purifying efficiency is high by adopting crystallization and recrystallization, and the used solvent is non-toxic.

Description

Technical field: [0001] The invention relates to a technology for rapidly extracting resveratrol from mulberry leaves, belonging to the technical field of natural product chemistry. Background technique [0002] Resveratrol is a non-flavonoid polyphenolic compound containing a stilbene structure. It has two configurations, cis and trans, of which the trans is a stable structure and has a wider range of biological activities. In recent years, many scholars at home and abroad have conducted in-depth research on the biological functions of resveratrol, and the results show that resveratrol has many biological activities beneficial to human health, such as anti-tumor, anti-cardiovascular disease, and anti-oxidation. attention from the biomedical community. [0003] So far, resveratrol has been found in a variety of plants, such as Vitis genus and Vitis genus in the family Vitaceae, Arachis in the Fabaceae family, Veratrum in the Liliaceae family, and Polygonum genus in the Poly...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/68C07C37/84
Inventor 唐忠海
Owner LUXI XIAOYUAN BIOTECH
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