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Gynogenesis method of grass carp and application thereof

A technology for gynogenesis and grass carp, applied to the gynogenesis of grass carp and its application field, can solve the problem of artificially induced gynogenesis grass carp hatching rate and survival rate is not high, inappropriate sperm inactivation haploid egg diploidy It can solve problems such as handling and easily damage grass carp eggs, and achieve the effect of solving the bottleneck problem of broodstock, facilitating batch processing, and having great application value.

Active Publication Date: 2011-06-29
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

But so far, due to various reasons such as easy damage to grass carp eggs during gynogenesis, improper sperm inactivation, and improper treatment of diploid haploid eggs, the hatching rate and survival rate of artificially induced gynogenetic grass carp have been reduced. Not high, the cost of induction method is high and the process is complicated, so far there is no report of large-scale establishment of pure line population of gynogenetic grass carp

Method used

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  • Gynogenesis method of grass carp and application thereof
  • Gynogenesis method of grass carp and application thereof
  • Gynogenesis method of grass carp and application thereof

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Embodiment

[0017] Further describe the gynogenetic method of grass carp of the present invention in detail below in conjunction with accompanying drawing, specifically implement according to the following steps:

[0018] 1. Sperm inactivation: Squeeze out the semen of allotetraploid crucian carp that has reached sexual maturity into a petri dish, dilute the semen with Hank's solution, the volume ratio of Hank's solution to semen is 4:1, and put the diluted semen in a petri dish Distributed in a thin layer, put the petri dish containing the semen on a shaker with an ice pack for centrifugation at a speed of 40-50r / min, and place it under two ultraviolet lamps with a power of 15W for irradiation treatment. The distance of the semen is 10-12cm, and the irradiation time is 20-30min. During the microscopic examination, the swing of the tail of the sperm is weakened and 80%-90% of the sperm still have good swimming vitality. Store in a refrigerator at 4°C.

[0019] The formula of Hank's solut...

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Abstract

The invention discloses a gynogenesis method of grass carp and application thereof. The method comprises the following steps: taking the diploid sperms of a properly inactivated allotetraploid crucian carp as a stimulus to stimulate mature eggs of the grass carp, after activating for 2-3 min or fertilizing for 20-22 min, carrying out the water-cooling shock treatment for 10-12 min at 4-6 DEG C, and hatching and controlling the temperature by to obtain the gynogenesis grass carp restraining the discharge of a second polar body or the first cleavage. The method greatly improves the hatching rate and the survival rate of the gynogenesis diploid grass carp, simplifies the preparation process, and can prepare a gynogenesis grass carp colony with good characters and stable heredity.

Description

technical field [0001] The invention relates to a method for cultivating and cultivating fish and its application, in particular to a method for cultivating grass carp and its application. Background technique [0002] Grass carp (Ctenopharyngodon idellus) is one of the important freshwater economically farmed fish in my country. Because it is prone to infectious diseases such as hemorrhage, enteritis, gill rot, and red skin, the survival rate of fish species is not high. In recent years, many scholars have aimed at the problem of poor disease resistance of grass carp. Through traditional gynogenetic methods, inactivated carp sperm, etc. are used to activate grass carp eggs, and then double the chromosomes to screen for those with strong disease resistance. A rich experience has been accumulated in grass carp individuals with excellent traits, and the biological disease resistance basis of gynogenetic grass carp has been preliminarily demonstrated. But so far, due to variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军周工健刘筠肖亚梅罗凯坤张纯陶敏张虹肖俊彭亮跃
Owner HUNAN NORMAL UNIVERSITY
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