Enzyme electrode and enzyme sensor
An enzyme electrode, electrode technology, applied in instruments, scientific instruments, biochemical equipment and methods, etc., can solve the problems of poor repeatability, short service life, lack of stability of enzyme electrodes, etc., to prevent the change of the three-dimensional structure, excellent The effect of sensitivity, excellent stability
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Embodiment 1
[0069](1) Synthesis of mesoporous silica material 3 In Example 1, mesoporous silica material 3 was synthesized first. More specifically, 271.59 g of No. 1 water glass and 828.41 g of water were mixed, followed by heating at 80°C. Separately, 80 g of behenyltrimethylammonium chloride (DTMA-C1) was added to 1 L of water at 70°C. After the solution became completely transparent, 70 mL of triisopropylbenzene was added to the solution, and the solution was vigorously stirred by a homomixer for 30 minutes. The emulsified solution was immediately added to the water glass solution and stirred again for 5 minutes. To the solution was added 2N hydrochloric acid over about 1 hour and stirred at pH 8.5 for about 3 hours. After suction filtering this solution, it was disperse|distributed in 70 degreeC heated water, and it filtered. After the solution was dried at 45° C. for 3 days, it was calcined in an electric furnace at 550° C. for 6 hours to obtain a white powdery mesoporous silica ...
Embodiment 2
[0086] (1) Synthesis of mesoporous silica material 3 In Example 2, firstly, a film-like mesoporous silica material 3 filled with one-dimensional silica nanochannel aggregates was synthesized. More specifically, 1.0 g of PEG-P123 copolymer, 20 mL of ethanol, 2 mL of water, and 100 μL of concentrated hydrochloric acid were mixed, followed by reflux at 60° C. for 1 hour with stirring. Further, 2.1 g of tetraethyl orthosilicate (TEOS) was added to the solution, and the solution was refluxed at 60° C. for 2 hours. Subsequently, 4 mL of this solution was taken out and dropped into a porous anodized aluminum membrane (diameter: 47 mm, thickness: 0.6 μM, pore diameter: 0.1 μM). Subsequently, after vacuum filtration, it was dried in a desiccator at room temperature for 20 minutes. The film-like mesoporous silica material 3 was obtained by calcining in an electric furnace at 500° C. for 5 hours. The film-like mesoporous silica material 3 was measured by a transmission electron microsc...
Embodiment 3
[0104] (1) Synthesis of the mesoporous silica material 3 In Example 3, the mesoporous silica material 3 and the membranous mesoporous silica material 3 filled with one-dimensional silica nanochannel aggregates were first synthesized. More specifically, white powdery mesoporous silica material 3 having an average pore diameter of about 2.7 nm, 4.2 nm, 6.2 nm, and 8.2 nm was obtained by a method similar to Example 1 or the like. Hereinafter, the obtained mesoporous silica material 3 may be referred to as FSM. In addition, film-like mesoporous silica material 3 having an average pore diameter of about 8.2 nm, 12.2 nm, 17.8 nm, and 98.4 nm was obtained by a method similar to that of Example 2 or the like. Hereinafter, the obtained mesoporous silica material 3 may be referred to as a mesoporous film.
[0105] (2) Formation of Enzyme-Protein Complex C Subsequently, Enzyme-Protein Complex C is formed by immobilizing the enzyme 4 in the mesoporous silica material 3 . As the enzyme 4...
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