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Carbon and nitrogen-regulating gene and protein and modulation thereof

A gene and transcription factor technology, applied in the field of regulating agronomic traits in plants, can solve the problem that trans-acting factors have not been characterized

Inactive Publication Date: 2010-03-31
UNIVERSITY OF GUELPH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite increased knowledge of structural genes involved in carbon and nitrogen metabolism, trans-acting factors involved in the transcriptional regulation of C / N gene expression have not been characterized

Method used

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  • Carbon and nitrogen-regulating gene and protein and modulation thereof
  • Carbon and nitrogen-regulating gene and protein and modulation thereof
  • Carbon and nitrogen-regulating gene and protein and modulation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0454] Materials and Methods

[0455] plant growth conditions

[0456] Sphagnum moss and vermiculate (1:4) (SunGro Horticulture Canada Ltd. BC, Canada) were used to grow Kaybonnet plants of rice with a weekly addition of a nutrient solution containing varying amounts of nitrate until harvest. The nutrient solution contains 4mM MgSO 4 , 5mM KCl, 5mM CaCl 2 , 1mM KH 2 PO 4 , 0.1mM Fe-EDTA, 0.5mM MES (pH6.0), 9μM MnSO 4 , 0.7μM ZnSO 4 , 0.3μM CuSO 4 , 46μM NaB 4 o 7 and 0.2μM (NH 4 ) 6 Mo 7 o 2. For restrictive N conditions, a 3 mM N solution was used once a week until harvest. For sufficient N conditions, 10 mM N solution was used once a week for the first six weeks, switched to 5 mM N solution for another 6 weeks, and switched to 3 mM N solution until harvest. Plants were grown for the first four weeks in a growth chamber (16 hours of light (approximately 400 μmolm -2 the s -1 ) and in the dark at 22 to 24°C for 8 hours), followed by a week of short-day treatme...

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Abstract

The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar and nitrogen accumulation and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar and nitrogen accumulation in plants. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield and increased stress tolerance.

Description

field of invention [0001] The present invention relates to methods of modulating agronomic traits in plants by modulating the expression of GATA transcription factors in plant cells. Specifically, this GATA transcription factor was isolated from rice (Oryza sativa) and is important in chlorophyll synthesis, sugar accumulation, nitrogen status, stress tolerance, and grain yield, and finally regulates nitrogen Intake and total carbon metabolism. Background of the invention [0002] Improving the phytoagronomic characteristics of crops has been going on since the beginning of agriculture. Most of the land suitable for crop production is currently in use. As the human population continues to grow, improved crop varieties will be required to adequately provide us with food and feed (Trewavas (2001) Plant Physiol. 125:174-179). To avoid catastrophic famine and malnutrition, future crop cultivars will need to achieve improved yields using equivalent agricultural inputs. These c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10A01H1/00A01H1/04A01H3/00A01H5/00C07K16/16C12N15/82C12N15/29C12Q1/68G01N33/53
CPCG01N33/6872G01N33/5097C12N15/8238
Inventor S·罗斯坦Y-M·毕Y－M·毕
Owner UNIVERSITY OF GUELPH
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