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Free living nitrogen fixing bacterium MBC9 and application thereof

A technology of self-generated nitrogen-fixing bacteria and bacteria agent, applied in the direction of application, bacteria, microorganisms, etc., can solve problems such as poor stress resistance, few self-generated nitrogen-fixing bacteria, and unsuitability for desert ecosystems

Active Publication Date: 2016-03-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on nitrogen-fixing bacteria are still mainly symbiotic nitrogen-fixing bacteria, and there are very few reports on spontaneous nitrogen-fixing bacteria.
Moreover, these small amounts of known autogenous nitrogen-fixing bacteria have requirements on environmental conditions such as ambient temperature and humidity, and have poor stress resistance. They are mostly used in farmland or compost, and are not suitable for desert ecosystems with harsh environmental conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The strain was extracted from the surface layer of the desert ecosystem in Ningxia. The climate in this area is arid; the temperature difference between the year and day and night is large (Feng Wei, 2014); the ultraviolet rays are very strong, especially in summer, the ultraviolet intensity is as high as 200-533μW / cm 2 (Pu Aiping, et al., 2005), the radiation index is around level 8. In general, the radiation intensity is 70-130μW / cm 2 Under ultraviolet rays, the radiation time ranges from 15s to 30min, and the sterilization rate can reach more than 99% (Li Xiangyun, et al., 2006, Zhang Fengcai and Yuan Ersen, 1988, Zhang Fuyun and Kuangfan, 2005). The ultraviolet intensity and irradiation time of ordinary sterilization conditions are obviously lower than the ultraviolet intensity in Ningxia summer field, so the strain is highly resistant to ultraviolet rays.

[0031]Weigh 10g of the surface soil sample and put it into 100mL of sterile water with glass beads and let ...

Embodiment 2

[0036] The azototroph MBC9 was inoculated in the primary slant medium and cultured at 30°C for 4 days to obtain the primary strain; the primary strain was inoculated in the secondary liquid medium and cultured with shaking at 30°C for 3 days to obtain Secondary strains; inoculate the secondary strains in the tertiary solid medium, cultivate them at 30°C for 2 days to obtain the tertiary strains; contact the tertiary strains with peat and mix them to obtain the bacterial agent; make the bacterial agent self-generate The content of nitrogen-fixing bacteria MBC9 was 2.9×10 8 cfu / g, the pH value of the bacterial agent is 7.0.

[0037] Wherein, the formula of described primary slant medium is: KH 2 PO 4 0.2g / L, MgSO 4 0.2g / L, NaCl0.2g, CaCO 3 5.0g, mannitol 10.0g, CaSO 4 0.1g, pH7.0, 20g agar, 1000mL distilled water;

[0038] The formula of the secondary liquid medium is: KH 2 PO 4 0.2g / L, MgSO 4 0.2g / L, NaCl0.2g, CaCO 3 5.0g, mannitol 10.0g, CaSO 4 0.1g, pH7.0, distille...

Embodiment 3

[0042] Mix the bacterial agent prepared in Example 2 with the sand according to the mass ratio of 1:10 to obtain a mixture, and then sprinkle the mixture on the soil surface at the southern edge of the Mu Us Sandy Land. The dosage of the bacterial agent is about 500g of the bacterial agent / mu.

[0043] One year after the bacterial agent was inoculated on the bare sand surface without nitrogen fixation activity at the southern edge of the Mu Us Sandy Land, the nitrogen fixation activity could be detected on the surface layer, which was 210.4nmolC 2 h 2 m -2 h -1 , and soil available nitrogen is also 3.16mgkg -1 increased to 18.5mgkg -1 .

[0044] The natural environmental conditions in the Mu Us Sandy Land are very harsh. The main disasters include drought, strong ultraviolet rays, strong winds, sandstorms, dry and hot winds, frost, and hail, which are not conducive to the survival and reproduction of animals, plants, and microorganisms. The Mu Us Sandy Land belongs to a...

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PUM

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Abstract

The invention discloses a free living nitrogen fixing bacterium massilia plicata MBC9. The preservation number of the free living nitrogen fixing bacterium massilia plicata MBC9 is CGMCC No.10825, the preservation date is May 19, 2015, and the preservation institution is China General Microbiological Culture collection Center. The strain can still live, breed and have nitrogen fixation activity under drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation. A microbial agent prepared from the free living nitrogen fixing bacterium massilia plicata MBC9 can be applied to ecological systems with drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation, can increase nitrogen accumulation in the ecological systems and can perform the function of improving soil environments and the physiological activity of microorganisms and plants and increasing net primary productivity.

Description

technical field [0001] The invention belongs to the field of biological nitrogen fixation, and in particular relates to a self-generated nitrogen-fixing bacterium MBC9 and its application. Background technique [0002] Desert ecosystems account for 41% of the global area (Delgado-Baquerizo, et al., 2013), and their net primary productivity accounts for about 20% of terrestrial ecosystem productivity (Whittaker, 1975). In desert ecosystems, the lack of soil nitrogen nutrients severely limits the system's net primary productivity (Asner, et al., 2003, Crutsinger, et al., 2013, Reich, et al., 2006). Moreover, desertification is still developing, leading to land degradation and serious loss of nitrogen nutrients. [0003] The nitrogen input in the natural environment is mainly biological nitrogen fixation. Nitrogen fixation increases the available nitrogen content of the soil, supplements nitrogen for microorganisms and plants to promote their growth and metabolism, and increa...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00C09K105/00C12R1/01
Inventor 张宇清邵晨曦秦树高冯薇吴斌
Owner BEIJING FORESTRY UNIVERSITY
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