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Curtobacterium citreum for cassava nitrogen fixation and preparation method and application thereof

A lemon-colored, short technology, applied in the field of nitrogen-fixing bacteria in the field of microbial technology, can solve the problems of diminishing returns, environmental pollution, etc., and achieve the effect of increasing the accumulation of nitrogen

Active Publication Date: 2016-07-13
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the excessive dependence of my country's agriculture on nitrogen fertilizer will inevitably lead to the risk of diminishing returns and environmental pollution, such as soil acidification, salinization, water eutrophication, and global greenhouse effect.

Method used

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  • Curtobacterium citreum for cassava nitrogen fixation and preparation method and application thereof
  • Curtobacterium citreum for cassava nitrogen fixation and preparation method and application thereof
  • Curtobacterium citreum for cassava nitrogen fixation and preparation method and application thereof

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experiment example 2

[0064] The present invention is that the endogenous nitrogen-fixing bacteria A02 strain belongs to Actinobacteria (Actinobacteria), Curtobacterium, and Curtobacteriumcitreum. Deposit unit: General Microbiology Center (CGMCC), China Committee for Microorganism Culture Collection, deposit date: March 07, 2016, deposit number: 12181.

[0065] The biological bacterial agent in which the Brevella citrinus is the active ingredient of the present invention is a biological bacterial agent prepared from the above-mentioned bacterial strains through tube seed cultivation and liquid fermentation.

[0066] The preparation method of the biological bacterial agent of the present invention includes tube culture and liquid fermentation experiment methods. Specifically include:

[0067] A: Tube seed culture: Inoculate the A02 strain on the slant medium of the test tube, and culture it at a constant temperature of 28-32°C for 2-3 days to obtain it.

[0068] B: Liquid fermentation: Inoculate t...

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Abstract

The invention discloses curtobacterium citreum for cassava nitrogen fixation and a preparation method and application thereof.The preservation number of the curtobacterium citreum is CGMCC No.12181.A biological microbial agent with the curtobacterium citreum as an active ingredient is prepared after the curtobacterium citreum is subjected to strain culture and liquid fermenting cultivation.When the strain and the biological microbial agent containing the same are used at the seedling stage of cassava growth, the nitrogen content of the roots, stem and leaves of cassava can be remarkably increased, nitrogen accumulation at the early stage of cassava growth is promoted, and high application value is achieved with respect to cassava production.

Description

technical field [0001] The invention belongs to the field of nitrogen-fixing bacteria in the technical field of microbes, and in particular relates to a short bacillus citrus for cassava nitrogen fixation and a preparation method and application thereof. Background technique [0002] Plant endophytic nitrogen-fixing bacteria are a group of microorganisms that colonize plants and can jointly fix nitrogen with host plants. The use of endogenous nitrogen-fixing bacteria can convert free nitrogen in the atmosphere into nitrogen that plants can directly absorb and use, and then further transform into protein, nucleic acid and other macromolecular substances needed by plants to supply the needs of plant growth. From an agricultural point of view, the use of biological nitrogen fixation is one of the important ways to provide nitrogen fertilizer for crops. Since the 1980s, endophytic nitrogen-fixing bacteria have become a research hotspot at home and abroad because of their nitrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C05F11/08C12R1/01
CPCC05F11/08C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 何冰张晓顾明华温荣辉
Owner GUANGXI UNIV
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