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Card for fast testing residual pesticide

A technology of residual pesticides and rapid test cards, which is applied in the field of rapid test cards for detecting residual pesticides, can solve problems such as dependence, low detection sensitivity, and insufficient response, and achieve the effects of sensitive detection, good anti-interference ability, and wide application range

Inactive Publication Date: 2010-04-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, test strips, quick test cards, and membrane chips for detecting pesticide residues have defects such as requiring more precise temperature control, relying on artificial folding of enzyme sheets and substrate sheets, insufficient reaction, and low detection sensitivity. Can not really achieve fast, sensitive, simple and accurate detection

Method used

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  • Card for fast testing residual pesticide
  • Card for fast testing residual pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] figure 1 It is a schematic structural diagram of the quick test card of the present embodiment, which is composed of figure 1 It can be seen that

[0020] The structure of the quick test card of the present embodiment is: the quick test card includes two layers of plastic plates; two holes are arranged on one of the plastic plates 7, one is the sample injection hole 1, and the other is the observation hole 2, and the diameters of the holes are 0.5cm;

[0021] One side of the plastic plate is pasted with absorbent paper 5 and an enzyme strip 4 fixed with Drosophila melanogaster acetylcholinesterase, the size of the absorbent paper is: long 20mm * wide 20mm, and the size of the enzyme strip is: long 20mm * wide 12mm, The amount of Drosophila melanogaster acetylcholinesterase immobilized on the enzyme strip is 3U, and the gelatin (prepared by dissolving gelatin in water) with a mass fraction of 0.4-1.2% is used for pasting; the absorbent paper 5 is opposite to the sample...

Embodiment 2

[0028] The quick test card of the present embodiment has the same structure as the embodiment, and the difference is that the diameters of the sampling hole and the observation hole are 0.7cm; the amount of Drosophila melanogaster acetylcholinesterase fixed by the enzyme strip is 4U; The amount of indoxyl acetate immobilized on the sheet was 8 mg; pasting was performed using 0.7% gelatin.

[0029] The process of using the test card to detect pesticide residues in samples is as follows:

[0030] Step 1, prepare a blank sample solution, the blank sample solution is 0.1mol / L PBK, its specific preparation process is as follows: take 12.48g dipotassium hydrogen phosphate, 3.85g potassium dihydrogen phosphate, dissolve in double distilled water to 1L, Adjust the pH value to 7.2 to obtain 1L of 0.1mol / L PBK;

[0031] Step 2: Add 0.5mL blank sample solution into the sample well, react at room temperature for 6 minutes, remove the baffle, and observe the color development result in th...

Embodiment 3

[0034] The quick test card of the present embodiment has the same structure as the embodiment, the difference is that: the diameter of the sample hole and the observation hole is 1cm; the amount of Drosophila melanogaster acetylcholinesterase fixed by the enzyme strip is 5U; the substrate The amount of indoxyl acetate immobilized on the sheet was 10 mg; pasting was performed using 1.2% gelatin.

[0035] The process of using the test card to detect pesticide residues in samples is as follows:

[0036] Step 1, prepare a blank sample solution, the blank sample solution is 0.1mol / L PBK, its specific preparation process is as follows: take 12.48g dipotassium hydrogen phosphate, 3.85g potassium dihydrogen phosphate, dissolve in double distilled water to 1L, Adjust the pH value to 7.2 to obtain 1L of 0.1mol / L PBK;

[0037] Step 2: Add 0.5mL blank sample solution into the sample well, react at room temperature for 6 minutes, remove the baffle, and observe the color development result...

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Abstract

A card for fast testing residual pesticide belongs to the technical field of biological engineering, and comprises two layers of plastic plates, wherein one of the plastic plates is provided with a sampling hole and an observation hole, one face of the plastic plate is provided with water-absorbing paper and an enzyme bar for fixing drosophila melanogaster acetylcholine esterase, the water-absorbing paper opposites the sampling hole, the enzyme bar opposites the observation hole, and the water-absorbing paper is connected with the enzyme bar. A base sheet for fixing indoxyl acetic ester is bonded on the other plastic plate at the position corresponding to the enzyme bar, and a baffle is arranged between the base sheet and the enzyme bar. When the sample and the indoxyl acetic ester in the enzyme bar pre-react, the baffle is drawn out to make the enzyme bar and the base sheet contact. The card for fast testing residual pesticide has the advantages of wide application range, delicate testing and fast and simple operation.

Description

technical field [0001] The invention relates to a quick test card in the technical field of bioengineering, in particular to a quick test card for detecting residual pesticides. Background technique [0002] my country is the world's largest pesticide user and producer, among which organophosphorus and carbamate pesticides account for 70% of the highly toxic varieties. Pesticide residues in major vegetables, fruits and other agricultural products in my country are very serious. At the same time, these highly toxic pesticides have caused serious pollution to the ecological environment such as water bodies and soil due to improper use and drift loss. Therefore, there is an urgent need for rapid, sensitive and accurate detection methods and supporting products for pesticide residues and pollution in my country's food safety supervision and environmental monitoring. The test strip detection method based on the enzyme inhibition method has been used as the national statutory de...

Claims

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Application Information

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IPC IPC(8): C12Q1/46
Inventor 张大兵武爱波池陈森
Owner SHANGHAI JIAO TONG UNIV
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