Method for preparing molecular bar code system for describing and discriminating animals and plants and application thereof

Abstract

The invention discloses a method for preparing a molecular bar code system for describing and discriminating animals and plants, which comprises the following steps: (1) acquiring monomorphic or polymorphic stripe data of animal and plant materials obtained by single or various molecular mark technologies and forming a mark set as a molecular mark set for discriminating a species, a subspecies, a variant, a breed and an inbred line or a variant single strain; (2) converting and storing a primer, a monomorphic or polymorphic mark and an analyzing result used in the name and the molecular mark of the molecular mark set obtained by the animal and plant materials by a two-dimensional bar code technology and expressing in a bar code way. The invention also discloses an application of the molecular bar code system obtained by the preparation method. The molecular bar code system is used for authenticating, discriminating, identifying or authorizing the species, the subspecies, the variant, the breed and the inbred line or variant single strain or storing and updating the data in ways of a computer, a network, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a molecular barcode system for describing and identifying animals and plants (species, subspecies, varieties, varieties, inbred lines or mutant individual plants), and an application of the molecular barcode system. Background technique [0002] Effective identification and screening of crop varieties has never been as important as it is today. In addition to the fact that there are many kinds of traditional crops and the variety resources are very rich, it is also caused by the following factors. First, with the development of agricultural production, the genetic basis of many species has become narrower and narrower while breeding has become more and more effective. Secondly, due to the reasons of seed production technology and operating profit, there are many fake and inferior varieties in the market, including low-purity hybrids and unsalable ...

AI Technical Summary

Problems solved by technology

Secondly, due to the reasons of seed production technology and operating profit, there are many fake and inferior varieties on the market, including low-purity hybrids and unsalable varieties that are shoddy and pretend to be good varieties.

Third, it is not uncommon to use high-quality hybrids with high market share for separation and purification, and illegal operation and theft of varieties are also common.

Although molecular markers are also used in the study of biological evolution, phylogeny, and animal and plant classification, the molecular markers currently used are of a single type and the number is not large, so they can only be limited to the classification or varieties of a few species in a certain group in the identification of animals and plants, but has not been widely used in the classification and identification of a wide range of animals and plants

Moreover, the defect of most molecular markers to distinguish species is that the data used for data analysis are qualitative data, and the types and numbers of markers used do not have a set of standards, which cannot be used for researchers to classify or identify new species, close relatives or recently differentiated species Provides directly available and shareable data information, and therefore is not included by researchers in the category of DNA "barcodes" that distinguish species above

Images