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Plant low temperature growth associated protein, code genes and application thereof

A gene and plant technology, which is applied to the low-temperature growth-related proteins of plants and their encoded genes and application fields, can solve the problems of low-temperature growth gene isolation and cloning that have not been reported, and achieves the ability to improve low-temperature tolerance and improve low-temperature tolerance. Effect

Inactive Publication Date: 2010-05-05
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a gene that affects germination at low temperature has been reported in rice by using the near-isogenic line strategy, but the isolation and cloning of genes that control low temperature growth have not been reported yet.

Method used

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  • Plant low temperature growth associated protein, code genes and application thereof
  • Plant low temperature growth associated protein, code genes and application thereof
  • Plant low temperature growth associated protein, code genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Discovery of OsCKI1 and its coding gene

[0042] 1. Genetic analysis and preliminary mapping of genes controlling low temperature growth in rice

[0043] From a set of chromosomal segment replacement lines (there are 66 families in total) with Asominori as the genetic background carrying an IR24 insertion, a family CSSL13 was found to have a cold-sensitive phenotype, and this family carried an IR24 insertion on the long arm of the second chromosome . The F1 of CSSL13 crossed with the background parent Asominori showed the Asominori phenotype, which confirmed that the phenotype sensitive to low temperature was a recessive phenotype. By investigating the segregation ratio of 144 F2 plants, we found that 36 plants exhibited the same phenotype as CSSL13 (3:1 ratio; x 2 ≈2.68figure 1 shown in a).

[0044] The method of initial positioning is as follows:

[0045] (1) Extract the total DNA of the above-mentioned selected single plant as a template, the specific ...

Embodiment 2

[0085] Embodiment 2, the acquisition and identification of transgenic plants

[0086] 1. Construction of recombinant expression vector

[0087] The pMD18-OsCKI1 constructed in step 3 of Example 1 was digested with HindIII and SacI, and the fragment containing the OsCKI1 gene was recovered, and connected to the pGA1611 vector (uniprotNo: 245199, the network link is http: / / www.uniprot.org / taxonomy / 245199; GENBANKACCESSION NO.AY373338.1) to obtain the recombinant expression vector.

[0088] The recombinant expression vector was transformed into Escherichia coli DH5a competent cells. The plasmid was extracted for enzyme digestion and sequencing detection. The detection results showed that a recombinant plasmid containing the OsCKI1 gene was obtained, which was named pGA1611-OsCKI1 (the backbone vector was pGA1611, and the OsCKI1 shown in sequence 2 was inserted between the HindIII and SacI sites. Gene).

[0089] Second, the acquisition of recombinant Agrobacterium

[0090] pG...

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Abstract

The protein is the following protein (a) or (b): (a) protein consisting of amino acid sequences shown in the sequence 1 of a sequence table; and (b) protein derived from the sequence 1 by substituting and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1 and associated with plant low temperature growth. After the protein is transferred to the plant, the protein enables that the low temperature sensitivity of plants is changed to low temperature insensitivity, so the protein can be applied to plant hereditary improvement and other work to improve the low temperature tolerance of plants (such as rice). The gene can be transferred to a low temperature intolerant variety to improve the low temperature tolerance thereof for the application of breeding of early season rice in the middle and lower reaches of Yangtze River and rice in northern areas of China.

Description

technical field [0001] The invention relates to a plant low-temperature growth-related protein, its coding gene and application. Background technique [0002] Plants are very fragile organisms and are easily affected by the surrounding environment, among which temperature is one of the most important factors affecting plant growth and development. Plants encountering low temperature and chilling damage will affect all aspects of their growth and development, including failure to germinate normally, slow growth, low fertility and even no fruiting. Among them, the influence of low temperature on germination and the effect of low temperature on fertility are research hotspots in recent years and many articles have been reported. However, there are few reports on the effects of low temperature on the process of plant growth and development. [0003] Rice (Oryza sativa L.) is the most important food crop in my country. The annual planting area accounts for nearly one-third of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/00C12N15/11C12N15/82
Inventor 万建民陆广文苏宁吴伟勋程志军雷财林张欣王久林郭秀萍
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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