Extraction method of cactus polysaccharide and application thereof in cigarettes
A technology of cactus polysaccharides and cactus, applied in the fields of application, tobacco, and tobacco processing
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Embodiment 1
[0024] a. The fresh stems of cactus are washed, dethorned, peeled, and dried, and then the enzymes are eliminated at high temperature (that is, baked at 80-105°C for 3-5 minutes), and then dried at 40°C for 3 hours until the moisture content is 8 % after crushing it to 80 mesh;
[0025] B, get the cactus dry powder that step a gained, add water and extract 2 hours under the condition of 60 ℃, water: material (mass ratio)=50: 1, leaching solution centrifuges 15 minutes through high-speed centrifuge, and supernatant is filtered through 200 meshes cloth filter;
[0026] c. Concentrate the filtrate to 1 / 4 of the original volume, add it to 95% ethanol, make the volume fraction of ethanol in the system reach 70%, let stand for 4 hours at 4°C, centrifuge for 20 minutes in a high-speed centrifuge, collect the precipitate and Obtain cactus crude polysaccharide;
[0027] d. Dissolving the cactus crude polysaccharide obtained in step c with water, centrifuging to remove impurities, and...
Embodiment 2
[0032] a. The fresh stems of cactus are washed, dethorned, peeled and dried, and then the enzymes are eliminated at high temperature, that is, baked at 80-105°C for 3-5 minutes, and then dried at 50°C for 2.5 hours, until the moisture content is 6%. Afterwards, it is pulverized to 80 orders;
[0033] B, get the cactus dry powder that step a gained, add water extraction 1 hour under the condition of 80 ℃, water: material (mass ratio)=40: 1, extract liquid centrifuges 15 minutes through high-speed centrifuge, supernatant is filtered through 200 meshes cloth filter;
[0034] c. Concentrate the filtrate to 1 / 6 of the original volume, add it to 95% ethanol, make the volume fraction of ethanol in the system reach 70%, let it stand for 4 hours at 4°C, settle in a high-speed centrifuge for 15 minutes, collect the precipitate and Obtain cactus crude polysaccharide;
[0035] d, putting the cacti crude polysaccharide obtained in step c into water for redissolving, and preparing a solut...
Embodiment 3
[0040] a. The fresh stems of cactus are washed, thorn-removed, peeled, and dried, and then the enzymes are eliminated at high temperature, that is, at 80-105°C for 3-5 minutes, and then dried at 65°C for 2 hours, until the moisture content is 5%. It is crushed to 100 mesh;
[0041] B, get the cactus dry powder that step a gained, add water and extract 4 hours at 70 ℃ of conditions, water: material (mass ratio)=70: 1, leaching solution centrifuges 15 minutes through high-speed centrifuge, and supernatant is through 200 order filter cloths filter;
[0042]c. Concentrate the filtrate to 1 / 10 of the original volume, add it to 95% ethanol, make the volume fraction of ethanol in the system reach 80%, let it stand for 8 hours at 4°C, settle in a high-speed centrifuge for 20 minutes, and collect the precipitate. Obtain cactus crude polysaccharide;
[0043] d. Redissolve the cactus crude polysaccharide obtained in step c, centrifuge to remove impurities, and put it on a macroporous r...
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