Culture and reproduction method of Lagerstroemia fauriei Keohne tissues

A technique of tissue culture and Fuchsia, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of solving the shortage of resources and obtaining high-efficiency

Inactive Publication Date: 2010-05-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And about the research on tissue culture of Lagerstroemia genus, there are few reports at home and abroad.
Only see reports such as Yang Yanling take the crape myrtle (Lagerstroemia indica) tender stem as the exp...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] 1. Select 50 robust annual cuttings of Lagerstroemia flockii. Cut off the young shoots above the ground, remove the leaves, and use the stem segments as explants, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 15-20 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash 4 times with sterile water, shaking for 3-5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.5mg / L+NAA0.05mg / L+GA 3 0.1mg / L+agar 6.5g / l, sucrose 30g / l, and control the pH of the medium to 5.8-6.0 at the same time, inoculate 3 plants in each bottle, and inoculate 70 bottles in total. Under the condition of light intensity 2000lx, culture...

Embodiment 2

[0020] 1. Select 30 robust annual cuttings of Lagerstroemia flockii. Cut the aboveground part, remove the leaves, and use the stem segment as an explant, each stem segment has an axillary bud, about 2cm long. Rinse the explants with clean water, rinse once with washing powder, and sterilize with 84 disinfectant on a shaker for 15-20 minutes. After rinsing with running water for 20-30 minutes, sterilize with 75% ethanol for 50-60 seconds on a sterile operating table, immediately pour 0.1% mercuric chloride for 10 minutes, and finally wash with sterile water 4 times, shaking for 3-5 minutes each time. Both ends of the sterilized explant were excised by 0.5 cm to remove the injured part. Access induction medium MS+6-BA0.5mg / L+NAA0.05mg / L+GA 3 0.1mg / L+agar 6.5g / l, sucrose 30g / l, at the same time control the pH of the medium to 5.8-6.0, pick 3 plants in each bottle, a total of 50 bottles. Under the conditions of temperature 20-26°C, light time 16h / day, and light intensity 1600lx...

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Abstract

The invention provides a culture and reproduction method of Lagerstroemia fauriei Keohne tissues, which comprises the following steps: firstly sterilizing exophyte of stalk sections of Lagerstroemia fauriei Keohne, culturing in an inducing culture medium to carry out bud differentiation induction, culturing in the dark at controlled culture temperature, normally culturing under illumination, cutting off young tips of Lagerstroemia fauriei Keohne, subculturing in a successive transfer culture medium, and finally carrying out the rooting culture on the Lagerstroemia fauriei Keohne sterile seedlings in a rooting culture medium, thereby obtaining the Lagerstroemia fauriei Keohne sterile seedlings. The method can effectively solve the problem of resource shortage of Lagerstroemia fauriei Keohne.

Description

technical field [0001] The invention relates to a tissue culture propagation method of Lagerstroemia fauriei Koehne, belonging to the technical field of woody plant seedling propagation. Background technique [0002] Lagerstroemia fauriei Koehne. It belongs to Lagerstroemia family, Lagerstroemia genus Lagerstroemia, a small deciduous tree with a height of 3 to 5 meters. The trunk is smooth, brown-reddish brown (when the trunk peels off between July and August). Odd pinnate compound leaves, 5-9 leaflets, oval, leathery. Panicles grow on the top of the branches, the flowers are milky white, and the flowering period is June to July. The fruit is nearly spherical, and the fruiting period is September to October. Positive tree species, slightly shade-tolerant, like warm and humid environment, drought-resistant, like fertile, relatively moist, well-drained calcareous soil, more resistant to water and humidity. It is an excellent garden ornamental tree species integrating tree ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李乃伟陆小清汤诗杰李云龙郭忠仁
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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