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A kind of oat polypeptide and its preparation method and application

A technology of oat polypeptide and oat bran, which can be used in the application field of food or medicine, and can solve the problem of less utilization of oat bran eggs

Active Publication Date: 2012-02-08
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oat bran is a by-product of oat processing, and its protein content is very high. At present, the research on oat bran at home and abroad is mainly focused on dietary fiber, and the utilization of oat bran eggs is seldom.

Method used

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  • A kind of oat polypeptide and its preparation method and application
  • A kind of oat polypeptide and its preparation method and application
  • A kind of oat polypeptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the preparation of oat hypotensive peptide

[0024] 1. Preparation of oat antihypertensive peptide

[0025] The process flow chart of extracting oat antihypertensive peptide is as follows: figure 2 As shown, the specific steps are as follows:

[0026] 1. Oat bran treatment:

[0027] In order to obtain more oat hypotensive peptides, oat bran is selected from ingredients between 20 mesh and 60 mesh (can be crushed), and the protein content in it can reach 25% as determined by Kjeldahl method.

[0028] 2. Pretreatment:

[0029] Mix 80 g of oat bran from 20 mesh to 60 mesh described in step 1 with deionized water in a weight ratio of 1:8, adjust the pH value to 9.0, and perform ultrasonic pretreatment in a water bath at 55°C (ultrasonic frequency 50kHz, power 176W), The processing time is 40min.

[0030] 3. Enzyme treatment:

[0031] After pretreatment, the pH value of the liquid was determined to be 8.0-8.5, and alkaline protease (purchased from Sichua...

Embodiment 2

[0036] Embodiment 2, the preparation of oat hypotensive peptide

[0037] 1. Preparation of oat antihypertensive peptide

[0038] The process flow chart of extracting oat antihypertensive peptide is as follows: figure 2 As shown, the specific steps are as follows:

[0039] 1. Oat bran treatment:

[0040] In order to obtain more oat hypotensive peptides, oat bran is selected from ingredients between 20 mesh and 60 mesh (can be crushed), and the protein content in it can reach 25% as determined by Kjeldahl method.

[0041] 2. Pretreatment:

[0042] Mix 80 g of oat bran from 20 mesh to 60 mesh described in step 1 with deionized water at a weight ratio of 1:10, adjust the pH value to 9.5, and perform ultrasonic pretreatment in a water bath at 50 ° C (ultrasonic frequency 50 kHz, power 176 W), The processing time is 50min.

[0043] 3. Enzyme treatment:

[0044] After pretreatment, the pH value of the liquid was determined to be 8.5, and alkaline protease (purchased from Sichu...

Embodiment 3

[0049] Embodiment 3, the preparation of oat hypotensive peptide

[0050] 1. Preparation of oat antihypertensive peptide

[0051] The process flow chart of extracting oat antihypertensive peptide is as follows: figure 2 As shown, the specific steps are as follows:

[0052] 1. Oat bran treatment:

[0053] In order to obtain more oat hypotensive peptides, oat bran is selected from ingredients between 20 mesh and 60 mesh (can be crushed), and the protein content in it can reach 25% as determined by Kjeldahl method.

[0054] 2. Pretreatment:

[0055] Mix 80 g of oat bran from 20 mesh to 60 mesh described in step 1 with deionized water at a weight ratio of 1:12, adjust the pH value to 8.5, and perform ultrasonic pretreatment in a water bath at 50 ° C (ultrasonic frequency 50 kHz, power 176 W), The processing time is 30min.

[0056] 3. Enzyme treatment:

[0057] After the pretreatment, the pH value of the liquid was determined to be 8.0, and alkaline protease (purchased from S...

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Abstract

The invention discloses oat polypeptide, a preparation method thereof and application thereof. The preparation method for the oat polypeptide comprises the following steps: 1) mixing oat bran and water, adjusting the pH of the mixture to be between 8.5 and 9.5, and carrying out ultrasonic pretreatment at the temperature of between 50 and 60 DEG C; 2) adding alkaline protease into the pretreated liquid, stirring the liquid for 2 to 5 hours at the temperature of between 50 and 60 DEG C; and then, adding flavourzyme into the liquid, and stirring the liquid for 1 to 2 hours at the temperature of between 50 and 60 DEG C; and 3) centrifuging the liquid after the enzymolysis to obtain supernate, adding beta-glucanase into the supernate, reacting medium temperature amylase with diastatic enzyme for 2 to 4 hours, and filtering the liquid to obtain supernatant liquor; and adsorbing and purifying the supernatant liquor by macroporous adsorption resin to obtain oat polypeptide containing solution. The oat polypeptide has the activity of inhibiting ACE enzyme, and can be widely applied in the fields of antihypertensive health-care food and the like. The method has simple operation, and the purity and yield are higher.

Description

technical field [0001] The invention relates to an oat polypeptide and its preparation method and application, in particular to an oat polypeptide and its preparation method and its application in preparing food or medicine for inhibiting ACE activity and lowering blood pressure. Background technique [0002] Oats, Poaceae Avena family Avena genus, annual or perennial herb. The nutritional value of oats is rich and comprehensive. Its protein, fat, vitamin E, dietary fiber, calcium, magnesium, iron, phosphorus and other mineral elements are higher than other cereal crops. It has the functions of lowering blood fat, cholesterol, blood pressure, blood sugar and anti-oxidation. Oxidation and other biologically active functions. [0003] Angiotensin-I-convertingenzyme (ACE, EC 3.4.15.1) is a zinc-containing dipeptide carboxylase, which is widely distributed in mammalian tissues and plays a key role in blood pressure regulation. ACE plays an important role in the regulation of b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K2/00A23L1/305A61K38/02A61P9/12A23L33/18
Inventor 何聪芬王昌涛董银卯赵华韩扬
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY