Fish protein oligopeptide for lefteye flounder
A technology of fish protein and flounder, which is applied in the field of compound feed additives for seawater fish, can solve the problems of slow growth and poor palatability, and achieve the effects of promoting growth, improving palatability, and rich resources
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[0012] This specific embodiment adopts the following technical solutions:
[0013] Production of fish protein oligopeptides: freshly frozen Pacific pollock leftovers are minced by a meat grinder and then put into an enzyme reactor, heated to 95°C and kept at 10°C for min to sterilize, then mixed with water and protease, the ratio of material to water is 1 : 1, using Alcalase enzyme E / S=90AU+Flavourzyme enzyme E / S=15000LAPU compound enzyme enzymatic hydrolysis method, enzymatic hydrolysis temperature 55°C, initial pH=8.5, enzymatic hydrolysis for 3 hours, and then the enzymatic hydrolysis solution is heated to inactivate the protease , coarse filtration to separate bone spurs, and further three-phase centrifugation of the enzymatic solution to obtain soluble protein oligopeptide aqueous phase, insoluble protein precipitate and fish oil respectively. Part of the soluble protein oligopeptide aqueous phase is purified by 1K ultrafiltration membrane ultrafiltration and spray dried ...
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