Preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV
A prrsv-n-igy, antibody technology, applied in the biological field, achieves the effect of stable physical properties and reducing the occurrence rate of false positives
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 The preparation technology and application of the antibody agent of the present invention will be further described in conjunction with the implementation below.
 1 Materials and methods
 1.1 Antigen preparation
Referring to the "Molecular Cloning Experiment Guide" and the PRRSV N protein gene sequence published on GenBank (GenBank accession number: FJ175688), PCR amplified the N gene, introduced EcoR I / HindIII restriction sites, and cloned the amplified fragment into prokaryotic expression In the vector pET-30a(+), transform the competent E.coli BL21(DE3)pLysS strain, pick a single colony and identify it by PCR and sequencing; -1 After induction with IPTG for 4 hours, the expression product was extracted and detected by SDS-PAGE; the recombinant protein was purified and expressed by Ni-NTAAgarose affinity chromatography, immunogenicity was detected by Western blot and protein concentration was determined by Bradford method, and the purified pro...
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