Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV

A prrsv-n-igy, antibody technology, applied in the biological field, achieves the effect of stable physical properties and reducing the occurrence rate of false positives

Inactive Publication Date: 2010-06-02
ZHEJIANG UNIV
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to antigenic differences, PRRSV isolates from various regions are divided into European type (prototype strain is LV) and North American type (prototype strain is VR-2332). These two viruses have similar biological properties, but have obvious differences. Serological differences

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV
  • Preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV
  • Preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The preparation technology and application of the antibody agent of the present invention will be further described in conjunction with the implementation below.

[0026] 1 Materials and methods

[0027] 1.1 Antigen preparation

[0028]Referring to the "Molecular Cloning Experiment Guide" and the PRRSV N protein gene sequence published on GenBank (GenBank accession number: FJ175688), PCR amplified the N gene, introduced EcoR I / HindIII restriction sites, and cloned the amplified fragment into prokaryotic expression In the vector pET-30a(+), transform the competent E.coli BL21(DE3)pLysS strain, pick a single colony and identify it by PCR and sequencing; -1 After induction with IPTG for 4 hours, the expression product was extracted and detected by SDS-PAGE; the recombinant protein was purified and expressed by Ni-NTAAgarose affinity chromatography, immunogenicity was detected by Western blot and protein concentration was determined by Bradford method, and the purified pro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Titeraaaaaaaaaa
Relative molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to the research for virus antibody and provides a preparation for PRRSV-N-IgY antibody and application thereof in detecting PRRSV. The preparation for antibody comprises the steps of preparing antigen by amplifying N genes on protein gene sequence PCR of PRRSV-N and extracting animal immunity and specific yolk immunity globulin. By using the recombined PRRSV-N protein of an antigen immunity layer, the specific PRRSV-N-IgY antibody is prepared. The prepared specific PRRSV-N-IgY antibody can be applied to detecting IFA of PRRSV. Compared with the antibody of animals such as rabbit, rat, and the like, IgY has stable physical property and can obviously decrease the occurrence rate of false positive without activating mammal complement, without combining with mammal Fc receptor or germ and without nonspecific reaction with RF (rheumatoid factors).

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an anti-porcine reproductive and respiratory syndrome virus (porcine productive and respiratory syndrome virus, PRRSV) nucleocapsid protein (N) protein-specific yolk immunoglobulin (anti-PRRSV-N-IgY antibody) The preparation of and its application in the detection of PRRSV by indirect immunofluorescence detection (IFA) method. Background technique [0002] The main pathogen of porcine reproductive and respiratory syndrome (porcine productive and respiratory syndrome, PRRS) is porcine reproductive and respiratory syndrome virus (porcine productive and respiratory syndrome virus, PRRSV), which was first discovered in the United States in 1987. It mainly manifests clinically as abortion, Premature births, stillbirths, mummies, a gradual decline in the survival rate of piglets, and respiratory symptoms in bred pigs, etc. Bring catastrophic economic loss to pig industry. In 1991, Wensvo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/10C07K16/02G01N33/569
Inventor 李肖梁方维焕
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com