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Immunowakeup microsystem as well as preparation method and application thereof

A microsystem and microsome technology, which can be used in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of restricting the widespread use of BMT, high requirements for treatment facilities, and expensive treatment costs. , to achieve the effect of improving immune killing function, overcoming immune tolerance, avoiding risks and serious side effects

Inactive Publication Date: 2012-01-18
SHANGHAI WEIKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limited source of matched bone marrow, high requirements for treatment facilities, and high cost of treatment, the widespread use of BMT in clinical practice is limited.

Method used

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  • Immunowakeup microsystem as well as preparation method and application thereof
  • Immunowakeup microsystem as well as preparation method and application thereof
  • Immunowakeup microsystem as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of immune awakening microsystem

[0035] 1. Preparation of immune microsomes

[0036] 1. Preparation of encapsulated microsomes:

[0037] Polyethylene glycol lactic acid (PLG poly(D, L-lactide-co-glycolide)), RG503BoehringerIngelheim Mr2400050:50 (w / w), 6% w / v, added bovine serum albumin (RIA grade Sigma) containing 1% , and rIL-2 (Roche) pre-dissolved in the buffer solution were added to dichloromethane, stirred at a medium speed to form an emulsion (w / o), quickly added emulsifier polyvinyl alcohol 10% PVAw / v, stirred at high speed in an ice bath for 30 Minutes into an emulsion (w / o / w), stirred overnight at room temperature at low speed, and evaporated the organic solvent. After solidification, the microspheres were collected by centrifugation, washed three times with double distilled water, and dried in vacuum. The yield was greater than 70%. The microspheres are dispersed in distilled water by ultrasonic waves, one drop is placed on a glass slid...

Embodiment 2

[0055] Experiments on animal models of solid tumors and leukemia treated with IMS

[0056] 1. In the mouse OVA tumor experiment, 6-10 week old mouse C57BL / 6 was used as host recipient, and Balb / c was used as donor CD4+ cells to prepare IMS. OVA-expressing EG-7 cells10 6 Inject C57BL / 6 mice intradermally, and start treatment after the tumor reaches 5-7mm five days later. In the B-cell leukemia experiment, 6-10 week-old mouse Balb / c was used as host recipient, and C57BL / 6 was used as donor CD4+ cells to prepare IMS. When BCL2 cells 10E6 were injected into Balb / c mice, the spleen expanded to twice its original size one week later, and peripheral blood lymphocytes were destroyed and lysed before treatment. Donor CD4+ cells were isolated from splenocytes, activated and cross-linked immune microsomes, and IMS was prepared according to the steps in Example 1.

[0057] 2. Survival rate: After one week, 10E5 IMS was injected intravenously, and after two weeks, 10E5 IMS was injected ...

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Abstract

The invention relates to an immunowakeup microsystem as well as a preparation method and application thereof. The immunowakeup microsystem comprises a cell and an immune microsome, wherein the cell and the immune microsome are crosslinked to form an immune awakening microsystem integral structure by an antibody and an antigen; the cell is a CD4+T cell with a CD4OL expression, and the immune microsome is a microsome of biodegradable material loading recombination rIL-2. The invention simulates the mechanism that bone marrow transplantation (BMT) generates graft versus tumor (GVT) for the first time, constructs the immunowakeup microsystem based on allosome immunocompetence T cells and generates allosome immunoreaction and I type anticancer effect, which are the same with the BMT without the requirement of marrow matching and the serious side effect of graft versus host.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to an immune awakening microsystem including cells and immune microsomes and its preparation method and application. 【Background technique】 [0002] Bone marrow transplantation (BMT) can produce a robust graft-versus-cancer response (GVT) and is currently the only cure for blood cancers and other solid tumors. The application of BMT has a history of more than 30 years, but the anti-cancer mechanism has not been clear for a long time. The method of BMT to treat cancer is first to destroy the patient's bone marrow immune system with high-dose radiation and chemical drugs, and then transplant the donor's bone marrow through bone marrow matching. It is currently known that this BMT method of "reconstructing the immune system" is essentially an extremely effective anti-cancer immune response. GVT can continuously eliminate cancer cells until the last cancer cell disappears. [0003] Howe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/20A61K35/14A61K47/48A61P35/00A61K35/15A61K47/69
Inventor 唐申北
Owner SHANGHAI WEIKE BIOTECH