Technology for extracting dendrobium polysaccharide by applying biology engineering enzyme technology and product thereof and application
A technology of Dendrobium polysaccharides and biological preparations, applied in the field of separation and extraction of Dendrobium polysaccharides, can solve the problems of low extraction rate, high temperature, and high cost
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[0022] The present invention is illustrated below in conjunction with an extraction process embodiment of Dendrobium polysaccharide. The extraction process embodiment is the same as the following process steps.
[0023] The extraction process of dendrobium extract dendrobium polysaccharide comprises the following steps:
[0024] 1, the dendrobium plant after air-drying is pulverized;
[0025] 2. Put the crushed dendrobium powder in a square stainless steel container, and add 10 times the amount of water to mix;
[0026] 3. Add 2% cellulase to the mixture;
[0027] 4. Adjust the pH to 6, keep the water temperature constant at 40°C, and enzymatically hydrolyze for 2 hours;
[0028] 5. Water extraction 3 times after enzymatic hydrolysis, 1 hour each time
[0029] 6. After filtering the above three water extracts, mix them, put them in a reaction tank and concentrate them by rotary evaporation to a ratio of 1:2;
[0030] 7. Alcohol precipitation: Slowly add ethanol with a pur...
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