Method utilizing Co60-Gamma ray to carry out cold sterilization on Zilongjin medicine solution for cell culture
A drug solution and cell culture technology, applied in the direction of irradiation, etc., can solve the problems that the theory and method are not yet mature, and achieve the effect of strong versatility, non-toxic cost, and easy operation
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Embodiment 1
[0025] Example 1 Co60-γ-ray irradiation experiment plate counting simulated by Escherichia coli (DH5α)
[0026] The full steps include:
[0027] 1) Take 20ul of Escherichia coli (DH5α) glycerol-preserved strain and put it into 20ml 2YT medium, culture overnight, 37 degrees, 150rpm;
[0028] 2) Dilute the overnight cultured bacteria 1:10, dilute each 50ml with 2YT, and dilute 8 gradients of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , take 10 respectively -6 、10 -7 、10 -8 100ul each was used to smear the plate, and after 24 hours of incubation at a constant temperature of 37 degrees, counting;
[0029] 3) Take 3 copies of each diluted bacterial concentration gradient in parallel and send them to Tianjin Institute of Physics for Co60-γ-ray irradiation experiments. The irradiation time is 1h, 2h, 4h, and 8h;
[0030] 4) Take 100ul to smear on the plate, culture at 37°C for 24h, and then count.
[0031]
Embodiment
[0032] Example 2 Co60-γ-ray irradiation experiment plate counting simulated by Staphylococcus aureus
[0033] The full steps include:
[0034] 1) Take 20ul of Staphylococcus aureus preserved in glycerol and put it into 20ml of 2YT medium, culture overnight, 37 degrees, 150rpm;
[0035] 2) Dilute the overnight cultured bacteria 1:10, dilute each 50ml with 2YT, and dilute 8 gradients of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , take 10 respectively -6 、10 -7 、10 -8 100ul each was used to smear the plate, and after 24 hours of incubation at a constant temperature of 37 degrees, counting;
[0036] 3) Take 3 copies of the diluted bacterial concentration gradients in parallel and send them to Tianjin Institute of Physics for Co60-7 ray irradiation experiments. The irradiation time is 1h, 2h, 4h, 8h;
[0037] 4) Take 100ul to smear on the plate, culture at 37°C for 24h, and then count.
[0038]
Embodiment 3
[0039] Example 3 Escherichia coli (DH5α) simulated into Zilongjin solution for Co60-γ-ray irradiation Shot test shaker flask counting
[0040] The full steps include:
[0041] 1) Take 20ul of Escherichia coli (DH5α) glycerol-preserved strain and put it into 20ml 2YT medium, culture overnight, 37 degrees, 150rpm;
[0042] 2) Dilute the overnight cultured bacteria 1:10, dilute each 50ml with 2YT, and dilute 8 gradients of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , take 10 respectively -6 、10 -7 、10 -8 100ul each was used to smear the plate, and after 24 hours of incubation at a constant temperature of 37 degrees, counting;
[0043] 3) Take 3 copies of each diluted bacterial concentration gradient in parallel and send them to Tianjin Institute of Physics for Co60-γ-ray irradiation experiments. The irradiation time is 1h, 2h, 4h, and 8h;
[0044] 4) Take 100ul to smear the plate, and transfer the rest into a sterile Erlenmeyer flask, culture at 37°C, ...
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