Method for producing betanine with suaeda salsa seed
A technology for Suaeda salsa and betalain, which is applied in the directions of chemical instruments and methods, botanical equipment and methods, applications, etc., can solve the problem of inability to cultivate red-leaf Suaeda salsa plants, high collection and storage costs, and raw material yield. Restrictions and other problems, to achieve the effect of short cycle, high purity and simple production method
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Embodiment 1
[0014] The mature seeds of wild Suaeda salsa are collected, dried and stored in a cool and ventilated place for later use. Take the river sand, wash it, expose it to the sun for several days and disinfect it, then put it into a culture dish for later use. The thickness of the river sand should be 2-3cm. Sow the seeds of Suaeda salsa in a tray filled with river sand and cultivate them in a dark environment with a culture temperature of 25°C and a relative humidity of 60%. Spray 1 / 2 of the MS culture solution twice a day to keep the river sand content. The water volume is 60% of the maximum water holding capacity. Three days later, take out the sand tray, cut the red cotyledons of Suaeda salsa sprouts to extract betalain, and clean the river sand of hypocotyls and radicles for repeated use. At room temperature, fully grind the red cotyledons of Suaeda salsa sprouts and add 80% methanol aqueous solution, the ratio of solid to liquid is 1:10, shake well and use RCF
[0015] Cent...
Embodiment 2
[0017] Collect the mature seeds of artificially cultivated Suaeda salsa, dry them and store them in a cool and ventilated place for later use. Take the river sand, wash it, expose it to the sun for several days and disinfect it, then put it into a culture dish for later use. The thickness of the river sand should be 2-3cm. Sow the seeds of Suaeda salsa in a tray filled with river sand and cultivate them in a dark environment at a temperature of 30°C and a relative humidity of 60%. Spray 1 / 2 of the MS culture solution twice a day to keep the river sand content. The water volume is 60% of the maximum water holding capacity. Three days later, take out the sand tray, cut the red cotyledons of Suaeda salsa sprouts to extract betalain, and clean the river sand of hypocotyls and radicles for repeated use. At room temperature, after fully grinding the red cotyledons of Suaeda salsa sprouts, add 80% aqueous methanol solution, the ratio of solid to liquid is 1:20, shake well and centri...
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