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Method for producing betanine with suaeda salsa seed

A technology for Suaeda salsa and betalain, which is applied in the directions of chemical instruments and methods, botanical equipment and methods, applications, etc., can solve the problem of inability to cultivate red-leaf Suaeda salsa plants, high collection and storage costs, and raw material yield. Restrictions and other problems, to achieve the effect of short cycle, high purity and simple production method

Inactive Publication Date: 2010-06-23
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing extraction of betalain from Suaeda salsa mainly utilizes red leaves (such as the publication number is CN101204217A patent), and the red leaves of Suaeda salsa can only be formed in the special habitat of the sea, and red leaves cannot be cultivated under artificial conditions. For Suaeda salsa plants, the output of raw materials is limited, and the cost of collection and storage is high, so it is not convenient for annual production

Method used

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  • Method for producing betanine with suaeda salsa seed
  • Method for producing betanine with suaeda salsa seed
  • Method for producing betanine with suaeda salsa seed

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0014] The mature seeds of wild Suaeda salsa are collected, dried and stored in a cool and ventilated place for later use. Take the river sand, wash it, expose it to the sun for several days and disinfect it, then put it into a culture dish for later use. The thickness of the river sand should be 2-3cm. Sow the seeds of Suaeda salsa in a tray filled with river sand and cultivate them in a dark environment with a culture temperature of 25°C and a relative humidity of 60%. Spray 1 / 2 of the MS culture solution twice a day to keep the river sand content. The water volume is 60% of the maximum water holding capacity. Three days later, take out the sand tray, cut the red cotyledons of Suaeda salsa sprouts to extract betalain, and clean the river sand of hypocotyls and radicles for repeated use. At room temperature, fully grind the red cotyledons of Suaeda salsa sprouts and add 80% methanol aqueous solution, the ratio of solid to liquid is 1:10, shake well and use RCF

[0015] Cent...

Embodiment 2

[0017] Collect the mature seeds of artificially cultivated Suaeda salsa, dry them and store them in a cool and ventilated place for later use. Take the river sand, wash it, expose it to the sun for several days and disinfect it, then put it into a culture dish for later use. The thickness of the river sand should be 2-3cm. Sow the seeds of Suaeda salsa in a tray filled with river sand and cultivate them in a dark environment at a temperature of 30°C and a relative humidity of 60%. Spray 1 / 2 of the MS culture solution twice a day to keep the river sand content. The water volume is 60% of the maximum water holding capacity. Three days later, take out the sand tray, cut the red cotyledons of Suaeda salsa sprouts to extract betalain, and clean the river sand of hypocotyls and radicles for repeated use. At room temperature, after fully grinding the red cotyledons of Suaeda salsa sprouts, add 80% aqueous methanol solution, the ratio of solid to liquid is 1:20, shake well and centri...

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Abstract

The invention relates to a method for producing betanine with suaeda salsa seed, which comprises: sowing the suaeda salsa seeds in a cultivation container containing sand bed, placing the container for cultivation for 2 to 5 days under dark environment, clipping red seed leaf of the germ of the suaeda salsa, adding methanol solution after completely grinding, shaking uniformly and centrifuging, taking supernatant, and performing vacuum concentration through a vacuum rotary evaporator to obtain betanine powder. The raw material of the invention is easy to take and store, and can not be affected by seasons, the production method is simple, the period is short and the purity of the betanine is high.

Description

technical field [0001] The invention relates to a production method of betaine, in particular to a method for inducing germination of plant seeds and separating and purifying betalain from cotyledons of sprouts, belonging to the technical field of natural pigment production. Background technique [0002] At present, betalain is mainly extracted from flowers, fruits and some clonal reproductive organs of plants, such as red beet root, dragon fruit peel, etc. The collection of raw materials is limited by seasons and plant growth cycles. Existing extraction of betalain from Suaeda salsa mainly utilizes red leaves (such as the publication number is CN101204217A patent), and the red leaves of Suaeda salsa can only be formed in the special habitat of the sea, and red leaves cannot be cultivated under artificial conditions. For Suaeda salsa plants, the output of raw materials is limited, and the cost of collection and storage is high, so it is not convenient for annual production. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00A01G31/00
Inventor 王长泉
Owner SHANDONG UNIV OF TECH
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