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Homocysteine diagnosing/measuring reagent (kit) and homocysteine concentration measuring method

A technology of homocysteine ​​and cystine transhydrogenase, which is applied in the field of medical inspection and measurement, and can solve the problems of complicated operation, long test time, and large variation of test data.

Inactive Publication Date: 2010-06-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0101] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0102] Stabilizer 500mmol / L

[0103] Glutathione Homocystine Transhydrogenase 10000U / L

[0104] Glutathione oxidase 12000U / L

[0105] Peroxidase 30000U / L

[0106] Glutathione disulfide 5mmol / L

[0107] 4-amino antiarsenic 2mmol / L

[0108] Carbolic acid 10mmol / L

[0109] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0110] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, volume ratio of tested homocysteine ​​sample to reagent is 1 / 25, reaction direction is Positive reaction (rising reaction), the detection method is the endpoint method, the delay time is 0 minu...

Embodiment 2

[0113] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0114] Reagent 1

[0115] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0116] Stabilizer 50mmol / L

[0117] Glutathione disulfide 5mmol / L

[0118] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0119] Reagent 2

[0120] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0121] Stabilizer 500mmol / L

[0122] Glutathione Homocystine Transhydrogenase 10000U / L

[0123] Glutathione oxidase 12000U / L

[0124] Peroxidase 30000U / L

[0125] 4-amino antiarsenic 0.6mmol / L

[0126] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0127] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 546nm, test sub-wavelength 600nm, the volume ratio of the tested homocysteine ​​sample to t...

Embodiment 3

[0130] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0131] Reagent 1

[0132] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0133] Stabilizer 50mmol / L

[0134] Glutathione disulfide 5mmol / L

[0135] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0136] Reagent 2

[0137] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0138] Stabilizer 500mmol / L

[0139] Glutathione oxidase 12000U / L

[0140] Peroxidase 30000U / L

[0141] Dimethylaniline 2mmol / L

[0142] Reagent 3

[0143] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0144] Stabilizer 500mmol / L

[0145] Glutathione Homocystine Transhydrogenase 10000U / L

[0146] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly. Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main...

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Abstract

The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, glutathione disulfide, glutathione homocysteine transhydrogenase, glutathione oxidase, peroxydase, reduced form chromogen combinations and stabilizing agents. Through a series of enzymatic reaction, the colorless reduced form chromogen combinations are finally oxidized into colored dyestuff, so the content of the dyestuff can be measured by a visible light analyzer in the position with the main wave length between 400 nm and 700 nm, and the concentration of the homocysteine can be further and directly reflected.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD