Preparation process of effective part of flower of abelmoschus manihot-total flavone extractive and new use thereof
A technology of effective parts and hollyhock flowers, applied in the field of biomedicine, can solve the problems that there are no reports on anti-hepatitis B virus and liver protection research.
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Embodiment 1
[0011] Example 1 Preparation of effective fractions of total flavonoids from hollyhock flower
[0012] Add an appropriate amount of 80% ethanol to the hollyhock flower, extract once, extract for 2 hours, combine the extracts, filter, concentrate the filtrate into a concentrated solution with a relative density of 1.13-1.16 at 60°C, add an appropriate amount of water to sink for 24 hours, and filter with suction. Concentrate to the corresponding volume, pass through a macroporous adsorption resin column, use alcohol-water gradient elution, water elution 3 column volumes, 10% and 30% ethanol each elution 3 column volumes, 70% ethanol elution 1 column volume . The eluate is concentrated, dried and pulverized to obtain the total flavonoid extract of the marshmallow flower, and the total flavonoid content is 65%.
Embodiment 2
[0013] Example 2 Preparation of effective fraction capsules of total flavonoids of marshmallow flowers
[0014] Take 250g of the extract of effective fractions of total flavonoids from hollyhock flowers, 50g of magnesium stearate, pass through an 80-mesh sieve, mix well, dry granulate, fill 1000 capsules, and each capsule contains 250mg of effective fractions from total flavonoids from hollyhock flowers. Usage: 2 capsules each time, three times a day.
Embodiment 3
[0015] Example 3 Preparation of tablets of effective fractions of total flavonoids from hollyhock flowers
[0016] Take 250g of the effective part extract of total flavonoids of hollyhock flower and 45g of microcrystalline cellulose, mix well, wet granulate with ethanol, and pass through a 20-mesh sieve. Add 5 g of magnesium stearate, mix evenly, and press to form 1000 tablets with a tablet weight of 0.3 g / tablet, each containing 250 mg of active parts of total flavonoids from hollyhock flowers. Usage: 2 tablets each time, three times a day.
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