Method for immobilizing petroleum degradation bacteria by taking surface root hair of reed as vector

Abstract

The invention relates to a method for immobilizing petroleum degradation bacteria by taking surface root hairs of reeds as vectors, which intensifies the decontamination capacity of a natural wetland for a petroleum polluted water body. The method is characterized by comprising the following steps of: firstly, screening out purified petroleum degradation bacteria strains by soil polluted by petroleum; then inoculating the purified petroleum degradation bacteria strains to a liquid enriched medium so as to culture petroleum degradation bacteria turbid liquid; finally adding sodium alginate to the prepared petroleum degradation bacteria turbid liquid, mixing, and then uniformly spraying to the root hairs of the reeds so as to achieve immobilization 5 hours later, i.e. immobilizing the petroleum degradation bacteria on the surface root hairs of the wetland reeds. The invention has the advantages of extensive material sources, simple process, low cost and good purifying effect, can achieve very good degradation effect even if the petroleum degradation bacteria turbid liquid with lower concentration is used and can be popularized and used on a large area of a reed wetland.

Description

technical field

[0001] The invention belongs to the field of strengthening the cleaning function of natural wetlands, and in particular relates to a method for immobilizing petroleum-degrading bacteria using reed surface roots as carriers. Background technique

[0002] At present, more than half of my country's surface water bodies are polluted to varying degrees, seriously affecting people's production and life. There are many methods to purify polluted surface water bodies, which are mainly divided into physical purification, chemical purification and biological purification. Among them, the physical purification method uses physical and mechanical methods to artificially purify the polluted surface water body. This type of method is simple in process, but it is expensive and treats the symptoms rather than the root cause. The chemical purification method mainly refers to adding chemical agents to the polluted water body, and through the chemical reaction between the agen...