Method for machining nanometer channel on microfluid chip

Abstract

The invention discloses a method for machining a nanometer channel on a microfluid chip, which comprises the following steps of: firstly, producing a chip with a nanometer level channel of a mixed liquor injecting channel and a main channel; filling a mixed liquor-storing hole with mixed liquor of liquor PDMS with proper viscosity and curing agent; leading the mixed liquor to flow into the main channel along the mixed liquor injecting channel; immediately adding equal buffer solution into a buffer solution-storing hole when the distance from the mixed liquor to the lower wall surface of the main channel is 3-4mu m; slipping the cylinder-shaped PDMS into the mixed liquor-storing hole; sealing the mixed liquor-storing hole and the buffer solution-storing hole with transparent adhesive; and forming a nanometer level channel with a certain length at the lower wall surface closely near to the main channel after the PDMS is completely cured. The method is very simple, convenient and fast, can combine with different designs chip structures as required, realizes the nanometer channels with different structures, and naturally integrates the nanometer channels with the chip, thereby being a universal method.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a method for processing nanometer channels on a microfluidic chip. Background technique [0002] Due to the size effect of the nanochannel structure, the specific surface area effect and the special physical and chemical properties inside and outside the pipeline, the nanochannel technology provides a new method in the detection and separation of chemistry and biomolecules. With the emergence of a variety of nanochannel processing technologies, some related technologies have gradually gained certain applications in many fields such as medicine, environmental monitoring, and homeland security. [0003] At present, the relatively mature nanochannel processing technologies mainly include: using natural channels, such as biomembrane ion channels; channels artificially synthesized with various materials, such as carbon nanotubes, arrayed nanochannels; and based on electron ...

AI Technical Summary

Problems solved by technology

[0005] 1. The process is complicated and the cost is high

For example, nanochannel processing methods based on electron beam lithography, coherent etching, and self-assembly have complex processing procedures, expensive processing equipment, and chips that cannot be reused many times, resulting in high processing costs.

[0006] 2. Nanochannels cannot be easily integrated with existing microfluidic chips, which limits the wide application of nanochannel technology

Existing nanochannel processing technology is difficult to integrate nanochannels into PDMS chips

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