Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for measuring purine with ultraviolet spectrophotometer

A spectrophotometer and ultraviolet technology, applied in the field of animal science, can solve problems such as complex composition, and achieve the effects of high repeatability, simple operation and accurate numerical value.

Inactive Publication Date: 2010-07-14
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microorganisms in the freeze-dried sample of rumen microorganisms exist in the form of bacteria, and at the same time contain other substances, and the composition is relatively complex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring purine with ultraviolet spectrophotometer
  • Method for measuring purine with ultraviolet spectrophotometer
  • Method for measuring purine with ultraviolet spectrophotometer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of ultraviolet spectrophotometer of the present invention measures the method for purine, and the steps are as follows:

[0019] Step 1: Accurately weigh 0.5 g of fully dried (dried with a freeze dryer) chyme sample, or a sample of the isolated chyme microbial part, and place it in a 25ml test tube with stoppers and scales. Note: the sample must be dry , otherwise it will lead to incomplete nucleic acid hydrolysis;

[0020] Step 2: add 2.5ml perchloric acid (HClO 4 , 1M), tightly cap the bottle mouth, and then place it in a constant temperature water bath at 90-95°C for 1 hour and incubate for 1 hour. At this time, the sample is carbonized and black indurations are formed. Break the indurations to facilitate the reaction to complete;

[0021] Step 3: Add 17.5ml of acetic acid buffer (NH 4 h 2 PO 4 , 0.0285M), fully shake, tightly cap the bottle, put it in a constant temperature water bath at 90-95°C for 10-15 minutes, and then filter through Wha...

Embodiment 2

[0030] Embodiment 2: the method that the ultraviolet spectrophotometer of the present invention measures purine is the purine recovery rate ultraviolet spectrophotometer method, and the purine recovery rate ultraviolet spectrophotometer method and liquid chromatography comparative study are as follows:

[0031] 1 Preparation of samples

[0032] 1.1 Preparation of rumen microbial freeze-dried samples

[0033] Take 1 L of rumen content 2 hours after morning feeding. Filter through two layers of cotton cloth and store at 4°C filled with CO 2 1L jar with a stopper for 30 minutes. Use a pipette to suck up a homogeneous liquid in the middle that does not contain the heavier particles in the lower layer and the lighter particles in the upper layer. Centrifuge at 20,000×g for 20 minutes at 4°C. The spheres were washed with distilled water, centrifuged, and finally freeze-dried.

[0034] 1.2 Preparation of neutral detergent fiber

[0035] Medium-sized detergent fibers were prepar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for measuring purine with an ultraviolet spectrophotometer, which comprises the following steps: accurately weighing 0.5g of fully dried chyme sample (dried with a freeze dryer), and placing the chymus sample in a 25ml test tube with scales; adding 2.5ml of perchloric acid, and placing the mixture in a constant-temperature water bath of 90-95 DEG C, and culturing for 1 hour; then adding 17.5ml of acetic acid buffer, placing the mixture in a constant-temperature water bath of 90-95 DEG C again, culturing for 10-15 minutes, and filtering; taking 0.5ml of filtrate, transferring the filtrate into a centrifuge tube with the volume being 15ml, and centrifuging at a speed of 4000rpm for 15 minutes; culturing in the constant-temperature water bath of 90-95 DEG C for 30 minutes; and carrying out colorimetry with an ultraviolet spectrophotometer in the place 260nm away, thereby obtaining the purine concentration expressed by a RNA equivalent. The invention has the advantages of simple operation, high repeatability and accurate numerical values. The content of microprotein in the duodenal chyme is determined through measuring the purine content in the chyme with the ultraviolet spectrophotometer, thereby providing a methodological basis for determination of the protein content in the metabolism of dairy cows.

Description

(1) Technical field [0001] The invention relates to animal science, in particular to a method for measuring purine with an ultraviolet spectrophotometer. (2) Background technology [0002] The dietary duodenal metabolizable protein (Metablizable protein, MP) of dairy cows includes dietary rumen undegradable protein (RUP), rumen microbial protein (Microbial crude protein, MCP) and endogenous protein (Endogenous crude protein, ECP) three parts. Rumen microbial protein accounts for 60-80% of metabolic protein, so quantification of rumen microbial protein synthesis is very important. The purine nitrogen method proposed by Zinn and Owen (1986) has been adopted by some scholars because of its simplicity, quickness, low test cost and easy operation, but it has also been questioned by some scholars because of the relatively low recovery rate of purines (Perez et al, 1997 ; Makker et al, 1999; Gonzalaz et al, 2003, 2004). This method is based on the hydrolysis of rumen microorgani...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 单安山乔国华
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com