Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance

A technology of Arabidopsis and genes, applied in the field of genetic engineering, can solve problems that have not been reported and achieve the effect of improving drought resistance response

Inactive Publication Date: 2010-07-21
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a number of genes related to iron reduction and transport have been cloned and functionally verified, the genes directly involved in the regulation of iron deficiency-induced rhizosphere acidification have not been reported so far.

Method used

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  • Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance
  • Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance
  • Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening and Identification of IAR1 Mutants and Related Plants' Iron Utilization and Drought Response

[0029] Using far-infrared imaging technology, the Arabidopsis thaliana mutant iar1 with higher leaf temperature was first screened under drought conditions, and the mutant gene IAR1 was cloned and numbered AT1G70690; overexpression of the gene was induced, and iron-deficiency medium could inhibit The root growth of corresponding Arabidopsis plants was inhibited, and at the same time, obvious iron deficiency symptoms of chlorosis and yellowing of leaves were caused. Atomic absorption method was used to determine that under the condition of iron deficiency induction, the accumulation of iron ions in IAR1 overexpression plants can be reduced, and the obvious symptoms of iron deficiency in plants can be caused. H 2 o 2 under the condition of existence. (See figure 1 )

Embodiment 2

[0031] Analysis of the effect of IAR1 on iron deficiency-induced rhizosphere acidification

[0032] The overexpression plants obtained by constructing the pCAMBIA1205 complementary vector, combined with T-DAN insertion mutation and induced IAR1 overexpression plant analysis showed that the overexpression plants showed more sensitive iron deficiency symptoms than wild-type Arabidopsis on iron-deficient medium, The color development of the pH chromogenic medium indicated that the overexpression plants lacked the ability of iron-induced rhizosphere acidification of Arabidopsis thaliana, indicating that IAR1 may regulate the utilization of iron nutrients in plants by regulating the acidification of Arabidopsis rhizosphere.

Embodiment 3

[0034] Gene expression and localization analysis

[0035]Analysis of phosphorus deficiency, zinc deficiency, copper deficiency, calcium deficiency, and manganese deficiency in the medium showed that the IAR1 gene had transferable response characteristics to iron deficiency; semi-quantitative PCR analysis showed that the expression of the IAR1 gene could be suppressed by the iron deficiency in the medium. Inhibit and regulate the iron-deficiency-induced rhizosphere acidification reaction of plants; the promoter of IAR1 gene was amplified by PCR and the corresponding GUS vector was constructed to obtain corresponding transgenic plants. tube expression.

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Abstract

The invention relates to application of an arabidopsis gene IAR1 in the aspects of iron nutrient and drought resistance, which is characterized in that: rhizosphere acidulation genes IAR1 related to arabidopsis iron absorption are regulated under the condition of iron deficiency; and the expression of the IAR1 gene controls the capacity of arabidopsis rhizosphere acidulation and absorption and utilization of plants to ion irons, and influences iron deficiency resisting reaction and drought resistance capacity of the plants. Analysis of functions and effects of the IAR1 gene provides basis and premise for obtaining new varieties of transgenic iron high-efficiency crops and improving drought resisting reaction of the plants.

Description

technical field [0001] The invention relates to an application of the Arabidopsis gene IAR1, in particular to an application of the Arabidopsis gene IAR1 in plant iron nutrition utilization and drought resistance, and belongs to the technical field of genetic engineering. Background technique [0002] Iron is the earliest trace element discovered by people. It has been discovered for more than two thousand years. Iron has an irreplaceable function in the life activities of prokaryotes and eukaryotes existing on the earth. Iron is essential for any biological organism. It is an essential mineral nutrient element. Mild iron deficiency in plants will reduce chlorophyll synthesis and photosynthetic rate. In severe iron deficiency, chlorophyll synthesis stops, new leaves turn yellow, and biomass decreases significantly. Plant iron deficiency not only affects the growth and development of plants, but also causes crop yield reduction. Plants are the main source of iron nutrition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 宋纯鹏安国勇王红霞张霖丁鹏赵孝亮王棚涛刘浩
Owner HENAN UNIVERSITY
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