Tobacco processing active complex enzyme preparation for improving and enhancing tobacco quality and preparation method thereof

A compound enzyme preparation and tobacco technology, which is applied in the fields of tobacco, tobacco treatment, hydrolytic enzymes, etc., can solve the problems of complex enzymes, etc.

Active Publication Date: 2010-07-28
云南万芳生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the enzymatic system is a complex system. For tobacco leaves that are rebaked, fermented, and prepared, the process of removing waste or turning waste into flavor and quality involves a wide variety of substances, and the corresponding specific enzymes and synergistic enzymes are even different. for complicated

Method used

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  • Tobacco processing active complex enzyme preparation for improving and enhancing tobacco quality and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Except for the 3-5 upper leaves of the tobacco plants, the apex leaves were taken as the raw material for extracting chlorophyllase, collected and stored in the shade for 3 days, and the chlorophyllase enzyme activity was sampled and tested. The combined enzyme activity was similar to that of the apex leaves; pH8. Dissolve the PMSF protease inhibitor in 0 buffer solution, add the top branch leaf when the concentration is 0.01mol / L, the ratio of the top branch leaf mass to the buffer solution containing PMSF volume is 1g: 4mL, break up the homogenate, filter, collect the filtrate, and let it stand 1h; Centrifuge the resting solution at 3500r / min for 20min, take the supernatant, add (NH 4 ) 2 SO 4 , so that the final concentration of ammonium sulfate is 25%, slowly stirring and dissolving, standing for 2h, and then adding (NH 4 ) 2 SO 4 Make the final concentration 80%, let it stand overnight, then centrifuge at 15000r / min at 4°C for 30min, take the supernatant, add 1...

Embodiment 2

[0115] When mixing the stock solution of chlorophyllase and carotenoid oxidase, the protease is added to the protease inhibitor solution with a concentration of 0.1 mol / L in equal volume in advance, and then mixed with other components. Other steps are the same as in Example 1.

Embodiment 3

[0117] Add the buffer solution containing PMSF to the filter residue of extracting chlorophyllase and carotenoid oxidase for the first time, the ratio of filter residue mass to buffer solution is 1g: 5mL, stir for 2h, filter and squeeze the residue, combine the filtrate with the previous filtrate, and let it stand 1h, centrifuge the standing solution at 3500r / min for 20min, take the supernatant, add (NH 4 ) 2 SO 4 Extract chlorophyllase and carotenoid oxidase. Other steps are the same as in Example 1.

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Abstract

The invention relates to a tobacco processing active complex enzyme preparation for improving and enhancing tobacco quality and a preparation method thereof, relating to multienzyme complex preparation added before tobacco redrying. In the method, oxidation-reduction enzyme is mainly used to process precursor matter chromoplast having larger contribution rate on tobacco quality and protein degradation product preposing Maillard reaction. The proportion of the complex enzyme preparation is as follows: 12000 U / mL of glucose oxidase, 370 U / mL of chlorophyllase, 230 U / mL of carotinoid oxidase, 190 U / mL of nicotine dehydrogenase, PMSF inhibitor and EDTA complexing agent. The activity of protease in preparation condensed from a basic solution is equivalent to 36000 U / mL of neutral protease under the temperature of 40 DEG C. The preparation is diluted by 250 times to replace a secondary tobacco wetting agent, thus being capable of improving quality, promoting grade, stabilizing commodity shelf life, and winning warm praise from customers.

Description

technical field [0001] The invention belongs to the multi-enzyme compound preparation for improving the grade of tobacco added before rebaking of tobacco. Background technique [0002] Nicotiana (Nicotiana L.) Solanaceae plants, there are more than 100 species in the world, mainly distributed in America and Oceania, introduced to my country in the early 17th century, it is one of the main sources of finance and taxation in my country, especially in Yunnan Province pillar industry. The habit of smoking tobacco is not easy to get rid of. High-quality tobacco leaves require high smoking safety, pure aroma, mellow taste, comfortable and elegant aftertaste, and moderate strength. [0003] The alcoholization or fermentation methods of tobacco leaves are divided into natural fermentation and artificial fermentation. Before the 1980s in my country, artificial fermentation was the main method, and after that, natural aging of tobacco leaves was actively carried out. In this century...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/04C12N9/50A24B15/20
Inventor 韩伟何宝玉陈应昆
Owner 云南万芳生物技术有限公司
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