Anthocyanidin identification method

An identification method, anthocyanin technology, applied in the field of identification of natural pigments, can solve the problems of tediousness, very high equipment and technical requirements, etc., and achieve the effect of high precision, convenient rapid preliminary identification, and low equipment and technical requirements

Inactive Publication Date: 2010-08-11
SHANDONG UNIV OF TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the structure identification of anthocyanins generally uses techniques such as high performance liquid chromatograp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anthocyanidin identification method
  • Anthocyanidin identification method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1

[0014] (1) Take 3 mg of cornflower pigment sample containing o-hydroxyl structure, add 15 ml of methanol, and shake well;

[0015] (2) Take 3 ml of the methanol solution of anthocyanins prepared in (1) with a cuvette, scan with a UV-Vis spectrophotometer in the range of 220nm to 700nm, and use methanol as a reference. According to the scanning curve, the maximum absorption peak of visible light is obtained at Near 530nm;

[0016] (3) Take 5 ml of anthocyanin methanol solution prepared in (1), add 3 drops of 5% ACl 3 After the solution is fully shaken, take 3 ml, add it to the cuvette and repeat the scanning using the method in (2), according to the scanning curve, the maximum absorption peak of visible light has shifted to the long wavelength direction and moved to the vicinity of 580nm;

[0017] (4) Take 5 ml of anthocyanin methanol solution prepared in (1), add 3 drops of 5% ACl 3 After the solution, shake well, then add 3 drops of 15% HCl solution and ...

Example Embodiment

[0018] Example 2

[0019] (1) Take 5 mg of peony anthocyanin sample containing o-hydroxyl structure, add 15 ml of methanol, and shake well;

[0020] (2) Take 3 ml of the methanol solution of anthocyanins prepared in (1) with a cuvette, scan with a UV-Vis spectrophotometer in the range of 220nm to 700nm, and use methanol as a reference. According to the scanning curve, the maximum absorption peak of visible light is obtained at Near 530nm;

[0021] (3) Take 5 ml of anthocyanin methanol solution prepared in (1), add 5 drops of 10% ACl 3 After the solution is fully shaken, take 3 ml, add it to the cuvette and repeat the scanning using the method in (2), according to the scanning curve, the maximum absorption peak of visible light has shifted to the long wavelength direction and moved to the vicinity of 580nm;

[0022] (4) Take 5 ml of anthocyanin methanol solution prepared in (1), add 5 drops of 10% ACl 3 After the solution, shake it well, add 5 drops of 20% HCl solution and s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an anthocyanidin identification method which uses an ultraviolet-visible spectrophotometer to scan methyl alcohol solution, methyl alcohol +ACl3 solution and methyl alcohol +ACl3 +HCl solution of anthocyanidin; and the anthocyanidins with single-hydroxy structure and neighbor-hydroxy structure are distinguished according to the change situation of maximum absorption peak position of a visible light area. The method is simple and easy, has lower requirement for equipment and technology, and is repaid in speed and high in accuracy.

Description

technical field [0001] The invention relates to an identification method of anthocyanidin, belonging to the technical field of identification of natural pigments. Background technique [0002] The anthocyanins in plants are all derived from six basic anthocyanins: geranium pigment, cyanidin, delphinidin, paeoniflorin, petunia pigment and mallow pigment. The structural difference of the six basic anthocyanins lies in the number and position of the hydroxyl group and methoxy group of the B ring. At present, the structural identification of anthocyanins generally uses techniques such as high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance, which are very cumbersome and require very high equipment and technology. Contents of the invention [0003] The object of the present invention is to provide a method for identifying the structure of anthocyanidin, which is simple and easy to implement, has low equipment and technical requirements, hig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31G01N21/33
Inventor 王长泉
Owner SHANDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap