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Anthocyanidin identification method

An identification method, anthocyanin technology, applied in the field of identification of natural pigments, can solve the problems of tediousness, very high equipment and technical requirements, etc., and achieve the effect of high precision, convenient rapid preliminary identification, and low equipment and technical requirements

Inactive Publication Date: 2010-08-11
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the structure identification of anthocyanins generally uses techniques such as high performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance, which are very cumbersome and require very high equipment and technology.

Method used

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  • Anthocyanidin identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Take 3 milligrams of cyanidin samples containing an adjacent hydroxyl structure, add 15 milliliters of methanol, and shake well;

[0015] (2) Use a cuvette to take 3 milliliters of the anthocyanin methanol solution in (1), scan it with a UV-visible light spectrophotometer in the range of 220nm to 700nm, use methanol as a reference, and obtain the maximum absorption peak of visible light according to the scanning curve at Around 530nm;

[0016] (3) Take 5 ml of anthocyanin methanol solution prepared in (1), add 3 drops of 5% ACl 3 Take 3 milliliters after the solution is fully shaken, add it to the cuvette and repeat the scanning by the method in (2), according to the scanning curve, the maximum absorption peak of visible light shifts to the long wavelength direction, and moves to around 580nm;

[0017] (4) Take 5 ml of anthocyanin methanol solution prepared in (1), add 3 drops of 5% ACl 3 Shake the solution well, then add 3 drops of 15% HCl solution and shake well...

Embodiment 2

[0019] (1) Take 5 mg of a sample of peony anthocyanin containing an o-hydroxy structure, add 15 ml of methanol, and shake well;

[0020] (2) Use a cuvette to take 3 milliliters of the anthocyanin methanol solution in (1), scan it with a UV-visible light spectrophotometer in the range of 220nm to 700nm, use methanol as a reference, and obtain the maximum absorption peak of visible light according to the scanning curve at Around 530nm;

[0021] (3) Take 5 ml of anthocyanin methanol solution prepared in (1), add 5 drops of 10% ACl 3 Take 3 milliliters after the solution is fully shaken, add it to the cuvette and repeat the scanning by the method in (2), according to the scanning curve, the maximum absorption peak of visible light shifts to the long wavelength direction, and moves to around 580nm;

[0022] (4) Take 5 ml of anthocyanin methanol solution prepared in (1), add 5 drops of 10% ACl 3 Shake the solution well, then add 5 drops of 20% HCl solution and shake well, then tak...

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Abstract

The invention relates to an anthocyanidin identification method which uses an ultraviolet-visible spectrophotometer to scan methyl alcohol solution, methyl alcohol +ACl3 solution and methyl alcohol +ACl3 +HCl solution of anthocyanidin; and the anthocyanidins with single-hydroxy structure and neighbor-hydroxy structure are distinguished according to the change situation of maximum absorption peak position of a visible light area. The method is simple and easy, has lower requirement for equipment and technology, and is repaid in speed and high in accuracy.

Description

technical field [0001] The invention relates to an identification method of anthocyanidin, belonging to the technical field of identification of natural pigments. Background technique [0002] The anthocyanins in plants are all derived from six basic anthocyanins: geranium pigment, cyanidin, delphinidin, paeoniflorin, petunia pigment and mallow pigment. The structural difference of the six basic anthocyanins lies in the number and position of the hydroxyl group and methoxy group of the B ring. At present, the structural identification of anthocyanins generally uses techniques such as high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance, which are very cumbersome and require very high equipment and technology. Contents of the invention [0003] The object of the present invention is to provide a method for identifying the structure of anthocyanidin, which is simple and easy to implement, has low equipment and technical requirements, hig...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33
Inventor 王长泉
Owner SHANDONG UNIV OF TECH
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