Micro-ecological feed additive for enhancing color of pet fish and preparation method thereof
A micro-ecological feed and additive technology, applied in animal feed, animal feed, applications, etc., can solve problems that are not conducive to the healthy and sustainable development of ornamental fisheries, environmental damage, water pollution, etc., to improve ornamental value, good regulation, no drug residue effect
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Embodiment 1
[0012] Preparation method: Candida utilis, Enterococcus faecalis: Lactobacillus acidophilus: Bacillus subtilis= = 1:1:1:1, after mixing the above-mentioned strains, ferment at a constant temperature in the culture medium according to the mass percentage of 10-15%, and stir while passing oxygen. Cultivate for 12 hours at 35°C for 24 hours until the fermentation culture reaches a pH value of 2.5.
[0013] The culture medium is 1.5g of agar, 1g of yeast, 1g of beef extract, 2g of yeast extract, 35g of sucrose, 2.0g of defoamer (commercially available product, this embodiment is purchased from Meichen Co., Ltd.), 1g of peptone, distilled water 1000mL, pH3.0~5.0. The yeast extract is yeast extract powder (commercially available product, yeast extract powder in this example was purchased from Beijing Aoboxing Biotechnology Co., Ltd.).
[0014] The Candida utilis, Enterococcus faecalis, Lactobacillus acidophilus and Bacillus subtilis were proliferated and cultured in the following ...
Embodiment 2
[0021] Preparation method: Candida utilis, Enterococcus faecalis: Lactobacillus acidophilus: Bacillus subtilis= =1:2:2:1, mix the above-mentioned strains and ferment in the culture medium at a constant temperature of 15% by mass, and stir while passing oxygen. The initial temperature of the culture is 28°C for 24 hours, and 30°C for 24 hours. , Cultivate at 35°C for 12 hours until the fermentation culture reaches a pH value of 3. The culture medium is 0.5 g of agar, 0.5 g of beef extract, 2 g of yeast extract, 30 g of sucrose, 3.0 g of defoamer, 0.5 g of peptone, and 1000 mL of distilled water. pH3.0~5.0, autoclave at 110~130℃. The yeast extract is yeast extract powder.
[0022] Application example
[0023] Select the market conventional species of ornamental fish red swordfish as the test material, use "Shishenhong" bait (purchased from Haifeng Feed Co., Ltd.) as the basic bait, add the color-enhancing feed additive obtained above at a ratio of 1‰ of the ornamental fish ba...
Embodiment 3
[0025] Preparation method: Candida utilis, Enterococcus faecalis: Lactobacillus acidophilus: Bacillus subtilis= = 3: 1: 1: 1, after mixing the above strains, ferment at a constant temperature in the culture medium according to the mass percentage of 12%, and stir while passing oxygen. The initial temperature of the culture is 28 ° C for 24 hours, and 30 ° C for 24 hours. , Cultivate at 35°C for 12 hours until the fermentation culture reaches a pH value of 3.5.
[0026] The culture medium is 1.5g of agar, 1.5g of beef extract, 2g of yeast extract, 45g of sucrose, 2g of defoamer, 1.5g of peptone, and 1000mL of distilled water. pH3.0~5.0, autoclave at 110~130℃.
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