Compound traditional Chinese medicine extractive composite for preventing and curing Alzheimer disease

A technology for Alzheimer's disease, compound traditional Chinese medicine, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of liver toxicity, can not delay the development of the disease, expensive drugs, etc., to improve memory, improve cognitive dysfunction , the effect of delaying development

Active Publication Date: 2010-08-18
PEKING UNIV FIRST HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The thinking of the Western medical circles to study anti-mild cognitive impairment and senile dementia drugs based on Alzheimer's disease mainly revolves around four anti-inflammatory (anti-inflammatory, anti-oxidation, anti-cholesterol, anti-β-amyloid protein) and two anti-( Currently, drugs approved by the US Food and Drug Administration for the treatment of Alzheimer's disease (AD) include tacrine, donepezil, rivastigmine, galant...

Method used

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  • Compound traditional Chinese medicine extractive composite for preventing and curing Alzheimer disease
  • Compound traditional Chinese medicine extractive composite for preventing and curing Alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, prepare the composition of total flavonoid extract and total polysaccharide extract

[0029] 1. Preparation of total flavonoid extract

[0030] 1) Weigh the following medicinal materials in parts by mass: Epimedium (6000 parts), fried dodder (6000 parts), medlar (6000 parts), raspberry (3000 parts), steamed schisandra (750 parts) and salt fried car Qianzi (1500 parts), after mixing, Chinese medicine composition is obtained.

[0031] 2) adding an aqueous ethanol solution with a mass concentration of 95% to the traditional Chinese medicine composition for extraction 3 times, the amount of alcohol added for the three extractions is 10 times the volume, 8 times the volume, and 6 times the volume successively, and the time for the three extractions is 1.5 hours. Combine three alcohol extracts and the remaining residue (for extracting total polysaccharides); extract the alcohol extract with petroleum ether, discard the ether phase after extraction, collect the ...

Embodiment 2

[0036] Example 2, the influence of the composition of compound total flavonoid extract and total polysaccharide extract on the learning and memory ability of dementia rats

[0037] 1. Materials

[0038] 1.1 Animals: SD male rats (SPF grade), weighing 285-315 g, provided by the Scientific Experimental Animal Center of the Chinese Academy of Military Medical Sciences (permit number SCXK-Jun-2007-004).

[0039] 1.2 Reagents and drugs: 1) Test drug: the composition of the compound total flavonoid extract and total polysaccharide extract prepared in Example 1; 2) The positive control drug is Huperzine A tablet, a product of Henan Tailong Pharmaceutical Co., Ltd., batch number: H10940156 ;3) Aβ 25-35 Purchased from Sigma Company, dissolved in sterile normal saline to prepare 5 μg / ml, aged at 37°C for 7 days, and stored at -20°C for later use.

[0040] 1.3 Experimental equipment: SN-3N stereotaxic instrument (Narishige, Japan), DMS-2Morris water maze system (Institute of Materia Me...

Embodiment 3

[0063] Example 3, the effect of compound total flavonoid extract and total polysaccharide extract composition on the long-term enhancement of dementia rats in vivo

[0064] 1. Materials

[0065] 1.1 Animals: Same as Example 3.

[0066] 1.2 Reagents and drugs: the same as in Example 3.

[0067] 1.3 Experimental equipment: SN-3N brain stereotaxic instrument (Narishige, Japan), VC-11 memory oscilloscope (Nihon Konden, Japan), SEN-7203 three-channel electronic stimulator (Nihon Konden, Japan), SS-202J stimulation isolator (Nihon Konden, Japan) Konden).

[0068] 2. Method

[0069] 2.1 Construction of AD model rats Same as in Example 4.

[0070] 2.2 The administration method is the same as that in Example 4.

[0071] 2.3 Experimental methods of electrophysiology

[0072] (1) Implantation of electrodes The animals were anesthetized with 20% urethane (urethane, 1.2g / kg, ip.) and fixed on a stereotaxic instrument. Anterior perforant path (PP) in the entorhinal area, its position...

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Abstract

The invention discloses a compound traditional Chinese medicine extractive composite for preventing and/or curing Alzheimer disease, which is composed of total formula flavone extractive and total formula polysaccharide extractive which are extracted from the following traditional Chinese medicine composite. The mass ratio of total formula flavone extractive to total formula polysaccharide extractive is 50-80:200-400; the traditional Chinese medicine composite is composed of the raw materials in parts by mass: 3000-12000 parts of herba epimedii, 3000-120000 parts of seed of Chinese dodder (fried), 3000-12000 parts of barbary wolfberry fruit, 1500-6000 parts of raspberry, 375-1500 parts of Schisandra chinensis (steamed) and 750-3000 parts of plantain seed (stir-heated with salt). Experiments prove that by the rat place navigation and spatial probe test, the total flavone extractive and the total polysaccharide extractive can improve the space learning memory ability of an AD (Alzheimer disease) model rat, shorten escape response latency and detection range, and obviously improve search time of quadrant on which the original platform is arranged in the spatial probe test as well as the percentage of space accounting for total time and space. Thus, the extractive composite can be used for preventing or curing the Alzheimer disease.

Description

technical field [0001] The invention relates to a compound traditional Chinese medicine extract composition for preventing and treating senile dementia. Background technique [0002] With the continuous acceleration of the pace of the world's entry into the aging society, various neurodegenerative senile diseases, such as mild cognitive impairment and Alzheimer's disease, have become important risk factors that threaten the health of human beings in their later years and reduce the quality of life of the elderly. The society and family have brought serious economic burden and heavy mental pressure. Therefore, the development of drugs for improving cognitive dysfunction and treating Alzheimer's disease has become the research focus of medical workers in various countries around the world, and has been highly valued by Chinese and Western medical circles. [0003] The thinking of the Western medical circles to study anti-mild cognitive impairment and senile dementia drugs bas...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61P25/28
Inventor 王学美黎巍威李娌曾克武富宏杨金霞张泰刘庚信
Owner PEKING UNIV FIRST HOSPITAL
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