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Rapid determination method for active component in mulberry tea or mulberry leaf

A technology for rapid determination of active components, applied in measuring devices, material separation, analysis of materials, etc., can solve problems such as complex process, detection failure, and small extraction volume

Inactive Publication Date: 2010-08-18
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of complex process of preparing active ingredients into essential oils in the existing pretreatment method of mulberry tea or mulberry leaves when using GC / MS mass spectrometer to identify and detect mulberry tea or mulberry leaves. It can not really reflect the composition of mulberry tea or mulberry leaves and the problem of expensive solid-phase microextraction method equipment, small extraction volume and frequent detection failures, but provides a rapid determination method for active components in mulberry tea or mulberry leaves

Method used

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  • Rapid determination method for active component in mulberry tea or mulberry leaf
  • Rapid determination method for active component in mulberry tea or mulberry leaf
  • Rapid determination method for active component in mulberry tea or mulberry leaf

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specific Embodiment approach 1

[0009] Specific embodiment one: the rapid determination method of active component in a kind of mulberry tea or mulberry leaf of the present embodiment is carried out according to the following steps: 1, mulberry tea or mulberry leaf is placed in airtight place 24h~28h in desiccator, then use The Tenax-TA adsorption tube filled with 200mg Tenax-TA is pumped and adsorbed for 5h~7h; 2. Connect the thermal desorption instrument to the GC / MS mass spectrometer, and put the Tenax-TA adsorption tube treated in step 1 into the thermal desorption instrument , set the desorption temperature of the thermal desorption instrument at 280°C to 320°C, and the desorption time at 5min to 7min, and use helium gas to purge the sample, and the sample injection time is 1min; For a TR-5MS capillary column with an inner diameter of 0.25mm and a film thickness of 0.25mm, the temperature program of the column is as follows: the column temperature is first raised to 40°C and kept for 1min, then raised to...

specific Embodiment approach 2

[0012] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the mulberry tea or mulberry leaves are placed in a desiccator and sealed for 25h-27h. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that: in step 1, the mulberry tea or mulberry leaves are placed in a desiccator and placed airtightly for 26 hours. Others are the same as in the first embodiment.

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Abstract

A rapid determination method for an active component in the mulberry tea or mulberry leaf relates to a determination method for an active component in the mulberry tea or mulberry leaf. The invention solves the problems that the process is complicated and the ingredients of the mulberry tea or mulberry leaf cannot be truly reflected in the method for preparing the essential oil from the active component in the mulberry tea or mulberry leaf, and the apparatus is expensive and the extraction content is small in the solid phase micro extraction method, which result in the test failure, in the existing pre-treatment method of the mulberry tea or mulberry leaf when testing and detecting the mulberry tea or mulberry leaf by a GC / MS (gas chromatography-mass spectrometer). The method comprises the following steps: 1, placing the mulberry tea or mulberry leaf in a dryer hermetically, aspirating and absorbing by a Tenax-TA absorption tube; 2, connecting a thermal desorption instrument with the GC-MS, placing the absorption tube in the thermal desorption instrument, setting parameters, and performing the determination; 3, calculating the relative content of the active component by a peak-area normalization method. The method is simple and free from property change of the active component, has low-cost apparatus and high detecting success rate up to 100%, and is capable of testing and detecting the mulberry tea or mulberry leaf.

Description

technical field [0001] The invention relates to a method for determining active components of mulberry tea or mulberry leaves. Background technique [0002] Mulberry leaf, also known as iron fan and silkworm leaf, is a plant of the Moraceae mulberry ( Morusalba The leaves of L.) are produced in most parts of the country. In addition to silkworm rearing, the mulberry leaves harvested at the first frost are removed from twigs and impurities, and dried in the sun to become traditional Chinese medicinal materials. The mulberry leaves can also be made into mulberry tea, which is used in beverages, health food, etc. [0003] Azulene and yohimbine are the main medicinal active ingredients in mulberry tea or mulberry leaves, where the molecular formula of azulene is: C 10 h 8 , its pharmacological effect is anti-ulcer; the molecular formula of yohimbine is: C 21 h 26 N 2 o 3 , which is a Rubiaceae plant yohimbe ( Pausinystaliayohimbe The alkaloids proposed in the dry bark o...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
Inventor 刘志明王海英
Owner NORTHEAST FORESTRY UNIVERSITY
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