Nitrogen-fixing gene, polypeptide and application thereof
A gene and nitrogen fixation technology, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as limitations, signal recognition, gene regulation and complex relationship, and achieve energy saving and environmental friendliness
Inactive Publication Date: 2010-08-25
SOUTH CHINA AGRI UNIV
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Problems solved by technology
The nitrogen fixation efficiency of symbiotic nitrogen-fixing bacteria is high, but it is limited to a few plants such as legumes
Method used
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The invention discloses a nitrogen-fixing gene, a polypeptide and application thereof. The nitrogen-fixing gene is provided with a DNA sequence as shown in SEQ ID NO 1, and the polypeptide is provided with an amino acid sequence as shown in SEQ ID NO 2. The invention adopts the method of reducing acetylene to ethylene, Nessler's reagent colorimetric method and 15N2 isotope labeling method so as to test, confirm and verify that the polypeptide has nitrogenase activity and can fix N2 in the air and reduce the N2 to NH4+. Therefore, the invention can be applied to industrial and agricultural production as a novel technology for biological nitrogen fixation.
Description
technical field The invention belongs to the technical field of biological nitrogen fixation, and specifically relates to a gene of Enterobacteriaceae, a polypeptide having the gene sequence, and an application of the gene and polypeptide in biological nitrogen fixation. Background technique The production of chemical nitrogen fertilizer is an energy-intensive process, while biological nitrogen fixation can use nitrogenase to fix nitrogen in the air into ammonia under normal temperature and pressure. The biological nitrogen fixation system itself is a small fertilizer factory, and the raw material comes from the air, which is inexhaustible. With the development of biotechnology and the improvement of research methods, the enzymes related to biological nitrogen fixation and the genes encoded by them are cloned, and genetic engineering operations are carried out to make the nitrogenase gene in prokaryotes. and expression in plant tissues, for energy saving, environmental impr...
Claims
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Login to View More IPC IPC(8): C12N15/31C12N15/10C12N15/70C07K14/265C05F11/08
Inventor 谭志远何玉梅彭桂香
Owner SOUTH CHINA AGRI UNIV