Recombinant BCG vaccine for tuberculosis prevention

A technology of recombinant BCG and BCG, applied in the direction of recombinant DNA technology, the use of vectors to introduce foreign genetic material, DNA / RNA fragments, etc., can solve problems such as safety concerns, and achieve excellent immunogenicity
CN101822829BInactive Publication Date: 2013-07-31SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN UNIV
Publication Date
2013-07-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to construction of a BCG vaccine through chimeric expression of GMCSF-CFP10-ESAT6 protein with a human GM-CSF gene, and a CFP10 gene and an ESAT6 gene of mycobacterium tuberculosis and research on immunogenicity of the BCG vaccine. In the construction method, a human GM-CSF gene sequence and a CFP10 gene sequence and an ESAT6 gene sequence of the mycobacterium tuberculosis are inserted into one escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV361 sequence through the genetic engineering technology to construct a recombinant shuttle plasmid rpMV361GMCSF-CFP10-ESAT6. Then the carrier is guided into a BCG vaccine through electroporation to construct a recombinant BCG vaccine rBCG: GMCSF-CFP10-ESAT6. The recombinant BCG vaccine constructed in the invention can stably express the GMCSF-CFP10-ESAT6 chimeric protein, and the immunogenicity of the recombinant BCG vaccine is superior to that of the immunogenicity of the traditional BCG vaccine. The inventionalso provides a preparation process of the recombinant BCG vaccine and research on the immunity of the recombinant BCG vaccine, which belong to the genetic engineering field and the tuberculosis vaccine field. Moreover, the invention can prevent the occurrence and spread of the tuberculosis more effectively.
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Description

Technical field:

[0001] The invention relates to the field of genetic engineering and the field of new tuberculosis vaccines, in particular to a recombinant BCG vaccine. Background technique:

[0002] Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. In recent years, with the increase of the floating population, the concurrent infection of human immunodeficiency virus (HIV) and Mycobacterium tuberculosis, and the emergence of multi-drug resistant strains of Mycobacterium tuberculosis , To increase the global tuberculosis epidemic situation, and present new challenges to global tuberculosis prevention and treatment. Bacille Calmette Guerin (BCG) is currently the only vaccine used to prevent tuberculosis, but its immune protection effect is extremely unstable. The immune protection effect of people in different regions after being vaccinated with BCG varies greatly (protection rate ranges from 0-80%) ). The development of a more effective anti-tuber...

Claims

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