High-strength temperature-sensitive hydrogel as well as preparation method and application thereof

Abstract

The invention relates to high-strength temperature-sensitive hydrogel as well as a preparation method and application thereof. The high-strength temperature-sensitive hydrogel is prepared by copolymerizing 2-vinyl-4,6-diamino-1,3,5-triazine and N-isopropyl acrylamide as raw materials in the presence of a cross-linking agent and an initiator, wherein the mass ratio of the 2-vinyl-4,6-diamino-1,3,5-triazine as a monomer to the N-isopropyl acrylamide is 0.2-2:1. The high-strength temperature-sensitive hydrogel has high stretching and compression resistance and favorable biocompatibility and optical property, and the surface of the hydrogel has the capacities of adsorbing DNA (Deoxyribonucleic Acid) transfection cells and regulating the attaching and disattaching behaviors of the cells through temperature. The preparation method of the hydrogel is simple, and the hydrogel is easy to store for a long term and transport for a long distance.

Description

technical field

[0001] The invention relates to a high-strength temperature-sensitive hydrogel and its preparation method and application, specifically a kind of N-isopropylacrylamide-2-vinyl-4,-diamino-1,3,5-triazine copolymer Preparation method and application of PNIPAAm-co-PVDT hydrogel, a high-strength temperature-sensitive hydrogel with surface-mediated gene transfection and cell detachment functions. Background technique

[0002] Hydrogel is a polymer material with cross-linked structure, which is hydrophilic but water-insoluble and can absorb a large amount of water (usually the water content is greater than 50% of the total mass) with water as the dispersion medium. Because of the physical crosslinking and chemical crosslinking between polymer chains, it will not dissolve in water, but can only swell and maintain a certain shape. At the same time, it also has good water permeability and biocompatibility. It can be used as a human implant Can reduce adverse reactions...

Examples

Embodiment 1

[0021]The monomer N-isopropylacrylamide (8mg), 2-vinyl-4,6-diamino-1,3,5-triazine (4mg) and the cross-linking agent polyethylene glycol diacrylate (8mg , molecular weight 575, the same below) was added to a 1.5ml centrifuge tube, after dissolving monomer and crosslinking agent with 200μl DMSO, adding photoinitiator Irgacure 2959 (0.4mg, 1-[4-(2-hydroxyethoxy )-phenylene]-2-hydroxy-2',2'-dimethylethanone). A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0022] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was designated PNV2-1. The size of the sample for the tensile mechanical property test...

Embodiment 2

[0025] The monomer N-N-isopropylacrylamide (7.2mg), 2-vinyl-4,6-diamino-1,3,5-triazine (4.8mg) and the cross-linking agent polyethylene glycol diacrylate (8 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and the cross-linking agent with 200 μl of DMSO, the photoinitiator Irgacure 2959 (0.4 mg) was added. A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0026] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was named PNV3-2. The size of the sample for the tensile mechanical property test is 20mm×2mm, and the thickness is 500μm. The sample size of the compression me...

Embodiment 3

[0029] Monomer N-isopropylacrylamide (6mg), 2-vinyl-4,6-diamino-1,3,5-triazine (6mg) and cross-linking agent polyethylene glycol diacrylate (8mg ) into a 1.5ml centrifuge tube, and after dissolving the monomer and the cross-linking agent with 200 μl of DMSO, the photoinitiator Irgacure 2959 (0.4 mg) was added. A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0030] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was designated PNV1-1. The size of the sample for the tensile mechanical property test is 20mm×2mm, and the thickness is 500μm. The sample size of the compression mechanical property ...

technical field

[0001] The invention relates to a high-strength temperature-sensitive hydrogel and its preparation method and application, specifically a kind of N-isopropylacrylamide-2-vinyl-4,-diamino-1,3,5-triazine copolymer Preparation method and application of PNIPAAm-co-PVDT hydrogel, a high-strength temperature-sensitive hydrogel with surface-mediated gene transfection and cell detachment functions. Background technique

[0002] Hydrogel is a polymer material with cross-linked structure, which is hydrophilic but water-insoluble and can absorb a large amount of water (usually the water content is greater than 50% of the total mass) with water as the dispersion medium. Because of the physical crosslinking and chemical crosslinking between polymer chains, it will not dissolve in water, but can only swell and maintain a certain shape. At the same time, it also has good water permeability and biocompatibility. It can be used as a human implant Can reduce adverse reactions...

Examples

Embodiment 1

[0021]The monomer N-isopropylacrylamide (8mg), 2-vinyl-4,6-diamino-1,3,5-triazine (4mg) and the cross-linking agent polyethylene glycol diacrylate (8mg , molecular weight 575, the same below) was added to a 1.5ml centrifuge tube, after dissolving monomer and crosslinking agent with 200μl DMSO, adding photoinitiator Irgacure 2959 (0.4mg, 1-[4-(2-hydroxyethoxy )-phenylene]-2-hydroxy-2',2'-dimethylethanone). A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0022] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was designated PNV2-1. The size of the sample for the tensile mechanical property test...

Embodiment 2

[0025] The monomer N-N-isopropylacrylamide (7.2mg), 2-vinyl-4,6-diamino-1,3,5-triazine (4.8mg) and the cross-linking agent polyethylene glycol diacrylate (8 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and the cross-linking agent with 200 μl of DMSO, the photoinitiator Irgacure 2959 (0.4 mg) was added. A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0026] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was named PNV3-2. The size of the sample for the tensile mechanical property test is 20mm×2mm, and the thickness is 500μm. The sample size of the compression me...

Embodiment 3

[0029] Monomer N-isopropylacrylamide (6mg), 2-vinyl-4,6-diamino-1,3,5-triazine (6mg) and cross-linking agent polyethylene glycol diacrylate (8mg ) into a 1.5ml centrifuge tube, and after dissolving the monomer and the cross-linking agent with 200 μl of DMSO, the photoinitiator Irgacure 2959 (0.4 mg) was added. A solvent containing monomers, crosslinking agents and initiators was injected into a closed mold, and the mold was irradiated in a UV curing box for 20 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water, soaked for 7 days, and replaced the deionized water every 12 hours.

[0030] Gel sheets were prepared by the same procedure for mechanical properties, optical properties and cell tests. This gel sample was designated PNV1-1. The size of the sample for the tensile mechanical property test is 20mm×2mm, and the thickness is 500μm. The sample size of the compression mechanical property ...

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