Fish embryo chromosome flaking method

Abstract

The invention discloses a fish embryo chromosome flaking method, relating to genetic engineering. The method includes the steps of taking out 100-200 roes during the middle-stage gastrula and the late-stage gastrula, washing the roes for twice through normal saline for fish to remove the membranes and the vitellines of the roes, adding the roes into colchicine-containing 1640 culture solution for cell culture, striking the roes to enable the cells to be even, adding fixative to fix the cells, drying and dyeing through staining mother solution. The invention overcomes the defects that the conventional method is time-consuming and labor-consuming and batch production can not carried out; and the fish embryo chromosome flaking method improves the stability of the flakes and the accuracy of the inductivity. Therefore, the fish embryo chromosome flaking method provides a reliable guarantee for genetic breeding and breed improvement of fish.

Description

technical field [0001] The invention relates to genetic engineering, in particular to a method for making fish embryo chromosome specimens. Background technique [0002] It is a development direction in the fishery to apply new technologies of bioengineering to fish genetic breeding and species improvement, especially fish cell engineering such as polyploid induction, which uses chromosomes as the object of operation, has shown broad application prospects . At present, the methods for ploidy detection of fish embryos mainly include single embryo preparation method and mixed embryo preparation method. The advantage of the single-embryo slice method is that it can accurately identify the ploidy of a single embryo. The disadvantage is that the operation is delicate, time-consuming and labor-intensive. When the number of embryos to be identified is large, the application of this method is limited. For example, when calculating the induction rate, at least More than 30 embryos ...

AI Technical Summary

Problems solved by technology

The advantage of the single-embryo slice method is that it can accurately identify the ploidy of a single embryo. The disadvantage is that the operation is delicate, time-consuming and labor-intensive. When the number of embryos to be identified is large, the application of this method is limited. For example, when calculating the induction rate, at least More than 30 embryos are needed, and the traditional method of mixed embryo production can solve the problem of large-scale production. However, when sampling, it is necessary to peel off the egg membrane and remove the yolk from each fish egg. The operation is very inconvenient and time-consuming. Embryo stage and gastrulation stage are the peak periods of embryo death. Excessive sampling time will cause a large number of samples to die, which not only affects the stability of the production results, but even leads to the failure of the experiment.