Method for measuring stable components in medicine by adopting gas chromatography/ mass spectrometry and efficient liquid chromatography

A high-performance liquid chromatography and gas chromatography technology, applied in the field of determination of stable components in traditional Chinese medicine, can solve the problems of affecting utilization and not found, and achieve the effect of avoiding precipitation, ensuring accurate qualitative and high-resolution characteristics

Inactive Publication Date: 2010-09-08
SHANXI INST OF COAL CHEM CHINESE ACAD OF SCI
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Problems solved by technology

But at present, the ingredients of many traditional Chinese medicines are unknown, which seriously affects its utilization.
In order to protect and develop the resources of traditional Chinese medicine in our country, it is necessary to

Method used

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  • Method for measuring stable components in medicine by adopting gas chromatography/ mass spectrometry and efficient liquid chromatography
  • Method for measuring stable components in medicine by adopting gas chromatography/ mass spectrometry and efficient liquid chromatography
  • Method for measuring stable components in medicine by adopting gas chromatography/ mass spectrometry and efficient liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Determination of Zaoren Anshen Granules

[0046] 1. Measuring instruments and implementation conditions:

[0047] ①.Waters Breeze 1525 high performance liquid chromatography, made in the United States, the instrument is equipped with Waters1525Binary high performance liquid chromatography pump, Dualλ2487 ultraviolet detector, the column type is VP-ODS SHIMADZU, 4.6x150mm, made in Japan, and three chromatographic columns are connected in series in the analysis Use, the chromatographic mobile phase is ethanol, the flow rate is 0.6 ml / min, the chromatographic column pressure is 2400PSI, the column temperature is 14°C, and the ultraviolet wavelength is selected at 254nm;

[0048] ② Finnigan Tracking Gas Chromatography / Mass Spectrometry (GC-MS) Analyzer is provided by American Finnigan Company, the conditions for gas chromatography / mass spectrometry analysis: the chromatographic column is a DB-5MS column, 30 meters long, 0.25 mm inner diameter, thin film The t...

Embodiment 2

[0071] Embodiment 2: the mensuration of Chinese medicine Passepartout

[0072] 1. Measuring instruments and implementation conditions:

[0073] ①.Waters Breeze 1525 high performance liquid chromatography, made in the United States, the instrument is equipped with Waters1525Binary high performance liquid chromatography pump, Dualλ2487 ultraviolet detector, the column type is VP-ODS SHIMADZU, 4.6x150mm, made in Japan, and three chromatographic columns are connected in series in the analysis Use, the chromatographic mobile phase is ethanol, the flow rate is 0.6 ml / min, the chromatographic column pressure is 2400PSI, the column temperature is 14°C, and the ultraviolet wavelength is selected at 254nm;

[0074] ② Finnigan Tracking Gas Chromatography / Mass Spectrometry (GC-MS) Analyzer is provided by American Finnigan Company, the conditions for gas chromatography / mass spectrometry analysis: the chromatographic column is a DB-5MS column, 30 meters long, 0.25 mm inner diameter, thin fi...

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Abstract

The invention relates to a method for measuring the stable components in medicine by adopting gas chromatography/mass spectrometry and efficient liquid chromatography through extracting solvent. The DB-5MS column and the chromatographic column which has a length of 30 m and an internal diameter of 0.25 mm are adopted by the gas chromatography and 3 chromatographic columns which are connected in series are adopted by the liquid chromatography so that the chromatographic analysis has the characteristic of high resolution and ensures the certainty. The reagent of ethanol, which has no toxicity to the human and causes no pollution to the environment, is adopted for the preparation of the sample. The ethanol is adopted by the mobile phase of the liquid chromatography so that the precipitation of the prepared sample is avoided and environment is not polluted during analysis. The method is used for measuring the stable components of the traditional Chinese medicine, which are very complex and very difficult to separate and identify.

Description

technical field [0001] The invention belongs to a method for determining stable components in traditional Chinese medicine, in particular to a method for determining stable components in traditional Chinese medicine by gas chromatography / mass spectrometry and high performance liquid chromatography. Background technique [0002] At present, there are many neurological disease patients in the world. Its manifestations are more serious anxiety, insomnia, convulsions, epilepsy and so on. They often need to use some tranquilizing drugs. There are dozens of drugs to treat neurological diseases. Among them, Western medicine is chemically synthesized, has a certain composition structure and relatively fixed analysis methods. my country is the birthplace of traditional Chinese medicine, which is rich in sources and often has good curative effects. However, the ingredients of many traditional Chinese medicines are unknown at present, which seriously affects its utilization. In or...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/36G01N30/72G01N30/06
Inventor 张昌鸣黄冬梅黄张根程秋霞
Owner SHANXI INST OF COAL CHEM CHINESE ACAD OF SCI
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