Method for measuring stable components in medicine by adopting gas chromatography/ mass spectrometry and efficient liquid chromatography

Abstract

The invention relates to a method for measuring the stable components in medicine by adopting gas chromatography / mass spectrometry and efficient liquid chromatography through extracting solvent. The DB-5MS column and the chromatographic column which has a length of 30 m and an internal diameter of 0.25 mm are adopted by the gas chromatography and 3 chromatographic columns which are connected in series are adopted by the liquid chromatography so that the chromatographic analysis has the characteristic of high resolution and ensures the certainty. The reagent of ethanol, which has no toxicity to the human and causes no pollution to the environment, is adopted for the preparation of the sample. The ethanol is adopted by the mobile phase of the liquid chromatography so that the precipitation of the prepared sample is avoided and environment is not polluted during analysis. The method is used for measuring the stable components of the traditional Chinese medicine, which are very complex and very difficult to separate and identify.

Description

technical field [0001] The invention belongs to a method for determining stable components in traditional Chinese medicine, in particular to a method for determining stable components in traditional Chinese medicine by gas chromatography / mass spectrometry and high performance liquid chromatography. Background technique [0002] At present, there are many neurological disease patients in the world. Its manifestations are more serious anxiety, insomnia, convulsions, epilepsy and so on. They often need to use some tranquilizing drugs. There are dozens of drugs to treat neurological diseases. Among them, Western medicine is chemically synthesized, has a certain composition structure and relatively fixed analysis methods. my country is the birthplace of traditional Chinese medicine, which is rich in sources and often has good curative effects. However, the ingredients of many traditional Chinese medicines are unknown at present, which seriously affects its utilization. In or...

AI Technical Summary

Problems solved by technology

But at present, the ingredients of many traditional Chinese medicines are unknown, which seriously affects its utilization.

In order to protect and develop the resources of traditional Chinese medicine in our country, it is necessary to establish some modern analysis methods and find out the components of traditional Chinese medicine. After searching, no literature reports on the determination method of stable components in traditional Chinese medicine have been found.

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